- Clone
- Poly9079 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Alpha-crystallin B chain, alpha B crystallin, heat shock protein beta-5, rosenthal fiber component, heat-shock 20 kD like-protein, renal carcinoma antigen NY-REN-27
- Previously
-
Covance Catalog# PRB-105P
- Isotype
- Rabbit Polyclonal IgG
-
Staining of Alpha B Crystallin, on formalin fixed paraffin embedded rat heart. -
Western blot of purified anti-alpha β crystalline antibody (clone Poly9079). Total tissue lysates (15 µg total protein) from human brain (lane 1), mouse brain (lane 2), and rat brain (lane 3) were resolved by 4-12% Bis-Tris gel electrophoresis, and then transferred to a PVDF membrane. The membrane was probed with 1.0 µg/mL of primary antibody overnight at 4°C, followed by incubation with HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:50000 dilution (lower). Lane M: Molecular weight marker.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 907903 | 25 µL | $89.00 | |||
Alpha B Crystallin (CryAB) is a member of the small heat shock protein (HSP20) family. Alpha crystallins are composed of two gene products: alpha-A and alpha-B, for acidic and basic, respectively. Alpha-A is preferentially restricted to the lens and alpha-B is expressed widely in many tissues and organs. CryAB can be induced by heat shock, ischemia and oxidation. The protein is known to protect cells from stress damage. It may also play an important role in the Golgi reorganization during cell division. CryAB is also known to be associated with a number of neurodegenerative diseases such as Alzheimer’s disease and has been implicated in breast tumors of patients with shorter survival, suggesting that it may be a useful marker in identifying aggressive breast cancer.
Product Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Formulation
- Phosphate-buffered solution + 0.03% Thimerosal.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1.0 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check your vial or your CoA to find the most accurate storage condition for this antibody.
- Application
-
IHC, WB
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For formalin-fixed paraffin-embedded immunohistochemical staining, a concentration range of 1 - 3 µg/mL is suggested. For western blotting, the suggested use of this reagent is 0.1 - 1 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
*Predicted MW = 22 kD
This product may contain other non-IgG subtypes. -
Application References
(PubMed link indicates BioLegend citation) -
- Thoua NM, et al. 2000. J Neuroimmunol. 104:47.
- Dasgupta S, et al. 1992. Exp Eye Res. 54:461.
- Bhat SP, et al. 1991. Euro J Biochem. 102:775.
- RRID
-
AB_2734684 (BioLegend Cat. No. 907903)
Antigen Details
- Biology Area
- Cell Biology, Cell Motility/Cytoskeleton/Structure, Neuroscience, Neuroscience Cell Markers
- Gene ID
- 1410 View all products for this Gene ID
- UniProt
- View information about Alpha beta Crystallin on UniProt.org
Other Formats
View All Alpha β Crystallin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Alpha β Crystallin | Poly9079 | IHC,WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Alpha β Crystallin
Staining of Alpha B Crystallin, on formalin fixed paraffin e... 
Western blot of purified anti-alpha β crystalline antibody (...