- Clone
- Poly28616 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GOT1, Aspartate Aminotransferase
- Isotype
- Rabbit Polyclonal IgG
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Western blot of purified anti-Aspartate Aminotransferase antibody (Poly28616). Lane 1: Molecular weight marker; Lane 2: 20 µg of human cerebellum lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled Donkey anti-Rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Aspartate Aminotransferase antibody (Poly28616) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 861601 | 25 µg | $125.00 | |||
GOT1 is the cytoplasmic form of aspartate aminotransferase and plays a role in amino acid metabolism, and the tricarboxylic acid cycles. GOT1 is one of the 6 genes that is commonly shared and regulated in the aging population and AD patients, suggesting that it may contribute as an aging-dependent AD risk factor1.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant Aspartate Aminotransferase (AST)
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 0.5 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810746 (BioLegend Cat. No. 861601)
Antigen Details
- Structure
- GOT1 is a 413 amino acid protein with an apparent molecular mass of ~ 46 kD.
- Distribution
-
Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Cytosol and nucleus - Function
- GOT1 is a cytoplasmic aspartate aminotransferase that plays a role in amino acid metabolism and the tricarboxylic acid cycles.
- Interaction
- Forms homodimer.
- Cell Type
- Astrocytes, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
- Biology Area
- Neurodegeneration, Neuroinflammation, Neuroscience
- Antigen References
-
- Zheng JJ, et al. 2018. Medicine (Baltimore). 97(28):e11343
- Zhang K, et al. 2018. Mol Carcinog. 57(11):1566
- Gene ID
- 2805 View all products for this Gene ID
- UniProt
- View information about Aspartate Aminotransferase on UniProt.org
Other Formats
View All Aspartate Aminotransferase Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Aspartate Aminotransferase | Poly28616 | WB,IHC-P |
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Purified anti-Aspartate Aminotransferase
Western blot of purified anti-Aspartate Aminotransferase ant... 
IHC staining of purified anti-Aspartate Aminotransferase ant...