Purified anti-Bcl-2 Antibody

Pricing & Availability
Clone
BCL/10C4 (See other available formats)
Regulatory Status
RUO
Other Names
B-cell leukemia 2
Isotype
Mouse IgG1, κ
BCL/10C4_WB_090908
Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/10C4.
  • BCL/10C4_WB_090908
    Western blot analysis of extracts from mouse splenocytes using anti-Bcl-2, clone BCL/10C4.
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Cat # Size Price Quantity Check Availability
633501 25 µg $101.00
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633502 100 µg $218.00
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Description

Bcl-2 (B-cell leukemia 2) is an apoptotic protein and a member of the Bcl-2 family containing BH1-4 domains. Two reported isoforms exist α=25 kD; β=22 kD. The Bcl-2 protein forms homo- or hetero-dimers with other Bcl-2 family members. Bcl-2 is distributed in the outer mitochondrial membrane, the nuclear envelope, and the endoplasmic reticulum. This protein blocks apoptotic death by controlling mitochondrial membrane permeability. Cleavage of Bcl-2 can convert to pro-apoptotic (by cleavage of BH4 domain). Bcl-2 has been reported to regulate cell cycle progression via ROS. This protein is modified by ASK1/JNK1, PKC, ERKs, and stress-activated kinase phosphorylation and can be ubiquitinated. Bcl-2 has been shown to interact with Apaf-1, Raf-1, TP53BP2, caspase-3, and form heterodimers with Bax, Bad, Bak, Bcl-xL, and Bag-1. Clone BCL/10C4 has been shown to be useful for Western blotting, immunoprecipitation, and immunofluorescence of the mouse and rat Bcl-2 protein.

Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
N-terminal, Amino acid residues 61-76 of mouse Bcl-2
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Upon receipt, store undiluted at between 2°C and 8°C.
Application

WB - Quality tested
IP, ICC, IHC-F - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, suggested working dilution(s): Use 5 µg per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: immunohistochemical staining of frozen tissue4, immunocytochemical staining5, and immunoprecipitation5.

Application References

(PubMed link indicates BioLegend citation)
  1. Hsu YT, et al. 1997. J. Biol. Chem. 272:13829.
  2. Zuber J, et al. 2011. Genes Dev. 25:1628. (WB) PubMed
  3. Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
  4. Yamanaka, et al. 2003. J. Immunol. 170:816. (IHC)
  5. Conus S, et al. 2000. EMBO J. 19:1534. (WB, IP, ICC)
Product Citations
  1. Wang HH, et al. 2022. J Biomed Sci. 29:36. PubMed
  2. Hailemichael Y, et al. 2022. Cancer Cell. 40:509. PubMed
  3. Zuber J, et al. 2011. Genes Dev. 25:1628. PubMed
  4. Kobayashi K, et al. 2020. Toxicol In Vitro. 63:104741. PubMed
  5. Wei SC, et al. 2019. Immunity. 50:1084. PubMed
  6. Tang J, et al. 2018. Front Immunol. 1.702777778. PubMed
  7. Doi K, et al. 2012. Biochem Biophys Res Commun. 425:107. PubMed
  8. Keller HR, et al. 2020. J Immunol. 204:3227. PubMed
  9. Sun Y, et al. 2020. J Immunol. 205:3358. PubMed
  10. Lai CC, et al. 2022. Biomed Rep. 16:19. PubMed
  11. Sochalska M, et al. 2015. Cell Death Differ. 10.1038/cdd.2015.130. PubMed
  12. Lee B, et al. 2019. Front Immunol. 0.561805556. PubMed
RRID
AB_961014 (BioLegend Cat. No. 633501)
AB_961014 (BioLegend Cat. No. 633502)

Antigen Details

Structure
Apoptosis regulator proteins, Bcl-2 family, BH1-4 domains. Homo- or hetero-dimer; isoforms α, β 25 kD, 22 kD
Distribution

Outer mitochondrial membrane, intracellular membrane nuclear envelope, endoplasmic reticulum

Function
Blocks apoptotic death by controlling the mitochondrial membrane permeability. Converted to pro-apoptotic activity by cleavage of BH4 domain. Regulates cell cycle progression via ROS
Modification
Phosphorylation, Ubiquitination
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
Antigen References

1. Tsujimoto Y, et al. 1986 P. Natl. Acad. Sci. USA 83:5214.
2. Yang E, et al. 1995. Cell 80:285.
3. Huang Z, et al. 2000. Oncogene 19:6627.
4. Deng X, et al. 2003. Blood. 102:3179.

Regulation
Phosphorylation by ASK1/JNK1, PKC, ERKs, stress-activated kinases
Gene ID
596 View all products for this Gene ID
UniProt
View information about Bcl-2 on UniProt.org
Go To Top Version: 3    Revision Date: 08/06/2019

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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