- Clone
- QA19A28 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- APP, Amyloid Beta (A4) Precursor Protein, AD1, CVAP, Peptidase Nexin-II, Alzheimer Disease Amyloid Protein, Cerebral Vascular Amyloid Peptide, Protease Nexin-II, ABPP, APP-I, PN-II, AAA
- Isotype
- Rabbit IgG
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IHC staining of anti-β-Amyloid, 1-38 antibody (clone QA19A28) on formalin-fixed paraffin-embedded human Alzheimer's disease (A-B) or normal (C) brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining. The images was captured with a 10X objective (A, C) and a 40X objective (B). -
IHC staining of anti-β-Amyloid, 1-38 antibody (clone QA19A28) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 88% formic acid for 20 minutes at room temperature, the tissue was incubated with the primary antibody at 10 µg/mL overnight at 4°C. BioLegend's Alexa Fluor® 594 donkey anti-rabbit IgG (minimal x-reactivity) antibody (Cat. No. 406418) was used for detection followed by DAPI counterstaining. The images were captured with a 40X objective. -
Synthetic human β-Amyloid peptides 1-38 (blue circles), 1-40 (Cat. No. 932601) (red squares), or 1-42 (Cat. No. 932501) (green triangles) were coated onto Costar™ 96-well, high binding assay plate at 2 µg/mL and then incubated with a dilution series of purified anti-β-Amyloid, 1-38 recombinant antibody (clone QA19A28). Bound antibodies were detected with HRP donkey anti-rabbit IgG antibody (Cat. No. 406401) followed by TMB substrate solution. Absorbance was measured at 450 nm. -
Synthetic β-Amyloid peptides 1-38, 1-40, and 1-42 (1 µg per lane) were run on a 16.5% Tris-Tricine gel, transferred to Nitrocellulose, and probed with 0.5 µg/mL of anti-β-amyloid, 1-38 recombinant antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rabbit IgG antibody (Cat. No. 406401) at a 1:3000 dilution. Lane M: Molecular weight marker.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 943301 | 25 µg | $129.00 | |||
| 943302 | 100 µg | $323.00 | |||
Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.
Product Details
- Antibody Type
- Recombinant
- Host Species
- Rabbit
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
IHC-F, Direct ELISA, WB - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 2.5 - 10 µg/mL is suggested. For immunohistochemistry, a concentration range of 5.0 - 10 µg/mL is suggested. Recommended usage concentration for direct ELISA application is 29 ng/mL. For western blotting, the suggested use of this reagent is 0.5 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is not recommended for WB application. It specifically recognizes β-amyloid peptide 1-38 but has poor sensitivity against the target.
- RRID
-
AB_2890865 (BioLegend Cat. No. 943301)
AB_2890865 (BioLegend Cat. No. 943302)
Antigen Details
- Structure
- Aβ 1-38 is 38 amino acid peptide with an expected molecular weight of 4 kD.
- Distribution
-
Extracellular
- Function
- Apoptosis, Cell Adhesion, Endocytosis, Notch Signaling
- Molecular Family
- APP/β-Amyloid
- Antigen References
-
1. Watts J, et al. 2014. Proc Natl Acad Sci U S A. 111:10323-10328.
2. Condello C, et al. 2018. Proc Natl Sci U S A. 115:E782.
3. Chen G, et al. 2017. Acta Phamacologica Sinica. 38:1205-1235. - Gene ID
- 351 View all products for this Gene ID
- UniProt
- View information about beta-Amyloid 1-38 on UniProt.org
Other Formats
View All β-Amyloid, 1-38 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-β-Amyloid, 1-38 Recombinant Antibody | QA19A28 | IHC-P,IHC-F,Direct ELISA,WB |
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Purified anti-β-Amyloid, 1-38 Recombinant Antibody
IHC staining of anti-β-Amyloid, 1-38 antibody (clone QA19A28... 
IHC staining of anti-β-Amyloid, 1-38 antibody (clone QA19A28... 
Synthetic human β-Amyloid peptides 1-38 (blue circles), 1-40... 
Synthetic β-Amyloid peptides 1-38, 1-40, and 1-42 (1 µg per ...