Purified anti-β-Amyloid, aggregated Antibody

Pricing & Availability
Clone
A17171C (See other available formats)
Regulatory Status
RUO
Other Names
Purified anti-β-Amyloid, AAA, ABETA, ABPP, AD1, APPI, APP, CTFgamma, CVAP, PN-II, PN2, Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide
Isotype
Rat IgG2a, κ
A17171C_PURE_beta-amyloid_Antibody_102418
IHC staining of purified anti-β-Amyloid, aggregated antibody (clone A17171C) on formalin-fixed paraffin-embedded normal human (left) and Alzheimer’s disease brain tissue (right). Following antigen retrieval using sodium citrate H.I.E.R, the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • A17171C_PURE_beta-amyloid_Antibody_102418
    IHC staining of purified anti-β-Amyloid, aggregated antibody (clone A17171C) on formalin-fixed paraffin-embedded normal human (left) and Alzheimer’s disease brain tissue (right). Following antigen retrieval using sodium citrate H.I.E.R, the tissue was incubated with 1 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The images were captured with a 40X objective. Scale bar: 50 µm
  • A17171C_PURE_beta-amyloid_Antibody_2_022520_updated.png
    Western blot of purified anti-β-Amyloid, aggregated antibody (clone A17171C). Lane 1: Molecular weight marker; Lane 2: 20 µg of Tris buffer (pH7.4) extract from Alzheimer’s disease brain; Lane 3: 20 µg of Tris buffer (pH 7.4) extract from normal human brain; Lane 4: 20 µg of Tris/2% SDS buffer extract from Alzheimer’s disease brain; Lane 5: 20 µg of Tris/2% SDS buffer extract from normal human brain. The blot was incubated with 5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP goat anti-rat IgG antibody (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
  • A17171C_PURE_beta-amyloid_Antibody_3_111219
    Western blot of purified anti-β-Amyloid, aggregated antibody (clone A17171C). Lane 1: Molecular weight marker; Lane 2: 2 µg of the human Aβ1-40 peptide; Lane 32 µg of the Aβ1-42 peptide; Lane 4: 25 ng of the recombinant human APP751 protein. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-rat IgG (Cat. No. 405405). Enhanced chemiluminescence was used as the detection system.
Compare all formats
Cat # Size Price Quantity Check Availability
856501 25 µg $129.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
856502 100 µg $323.00
Check Availability


Need larger quantities of this item?
Request Bulk Quote
Description

Alzheimer's disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor proteins (APP) through sequential proteolytic cleavage of APP by β-secretases and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Rat
Immunogen
Recombinant human Abeta S26C dimer.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

IHC-P - Quality tested
WB - Verified
Recommended Usage

Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/mL is suggested. For Western blotting, the suggested use of this reagent is 5.0 - 10 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2783458 (BioLegend Cat. No. 856501)
AB_2783458 (BioLegend Cat. No. 856502)

Antigen Details

Structure
Aβ denotes peptides of 36-43 amino acids generated from cleavage of APP by secretases. Aβ has an apparent molecular mass of about 4 kD.
Distribution

Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, and muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus.

Function
The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
Interaction
Tau, prion, α2-macroglobulin, apoE and apoJ, toll-like receptor 2 (TLR2), TLR4, TLR6, and their co-receptors CD14, CD36, and CD47.
Ligand/Receptor
Receptor for advanced glycation end products (RAGE), low-density lipoprotein receptor-related protein 1 (LRP1), glycoprotein 330 (gp330/megalin), P-glycoprotein.
Biology Area
Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
Molecular Family
APP/β-Amyloid
Antigen References
  1. Selkoe DJ. 1994. Annu. Rev. Cell Biol 10:373-403.
  2. O'Brien RJ, Wong PC. 2011. Annu. Rev. Neurosci. 34:185-204.
  3. Chen GF, et al. 2017. Acta. Pharmacol. Sin. 38:1205-1235.
Gene ID
351 View all products for this Gene ID
UniProt
View information about beta-Amyloid on UniProt.org
Go To Top Version: 3    Revision Date: 02/25/2020

8999 BioLegend Way, San Diego, CA 92121 www.biolegend.com
Toll-Free Phone: 1-877-Bio-Legend (246-5343) Phone: (858) 768-5800 Fax: (877) 455-9587

This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.

Login/Register
Forgot your password? Reset Password
Request an Account