- Clone
- 337.48 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, APP, Amyloid Precursor Protein, Pyroglutamate-modified Aβ, Pyroglutamate amyloid-β (Aβ), PyroGlu- Aβ, PyroGlu Abeta, Pyroglutamate-3 Amyloid-β
- Previously
-
Covance Catalog# MMS-5280
- Isotype
- Mouse IgG1, κ
-
IHC staining of purified anti-β-Amyloid Pyroglutamyl (Glu3) antibody (clone 337.48) on formalin-fixed paraffin-embedded Alzheimer's disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm -
IHC staining of purified anti-β-Amyloid Pyroglutamyl (Glu3) antibody (clone 337.48) on formalin-fixed paraffin-embedded Alzheimer's disease human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 822301 | 100 µL | $275.00 | |||
Amyloid beta (Aβ or Amyloid beta) denotes peptides of 36-43 amino acids in length that are crucially involved in Alzheimer's disease as the main component of the amyloid plaques found in the brains of Alzheimer patients. The peptides result from the amyloid precursor protein (APP), which is cut by certain enzymes to yield Aβ. Aβ molecules can aggregate to form oligomers (known as "seeds") which are believed to be able to induce other Aβ molecules to also take the misfolded oligomeric form, leading to a chain reaction akin to a prion infection. The seeds or the resulting amyloid plaques are toxic to nerve cells. The other protein implicated in Alzheimer's disease, tau protein, also forms such prion-like misfolded oligomers, and there is some evidence that misfolded Aβ can induce tau to misfold.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide corresponding to amino acids 3 through 16 of Amyloid Beta with a pyroglutamyl post translational modification at the third glutamate position (E3).
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2564857 (BioLegend Cat. No. 822301)
Antigen Details
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Gene ID
- 351 View all products for this Gene ID
- UniProt
- View information about beta-Amyloid Pyroglutamyl on UniProt.org
Other Formats
View All β-Amyloid Pyroglutamyl Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-β-Amyloid Pyroglutamyl (Glu3) | 337.48 | IHC-P |
Compare Data Across All Formats
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Purified anti-β-Amyloid Pyroglutamyl (Glu3)
IHC staining of purified anti-β-Amyloid Pyroglutamyl (Glu3) ... 
IHC staining of purified anti-β-Amyloid Pyroglutamyl (Glu3) ...