- Clone
- 1-11-3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Amyloid beta A4 protein, preA4, protease nexin-II, peptidase nexin-II, beta-amyloid peptide, alzheimer disease amyloid protein, cerebral vascular amyloid peptide, APP, Amyloid Precursor Protein
- Previously
-
Covance Catalog# SIG-39169
- Isotype
- Rabbit IgG
-
IHC staining of purified anti-β-Amyloid, x-42 antibody (clone 1-11-3) on formalin-fixed paraffin-embedded human Alzheimer's disease brain tissue. Following antigen retrieval using 70% formic acid for 20 minutes at room temperature, the tissue was incubated with 5 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 812101 | 50 µg | $431.00 | |||
Alzheimer’s disease is characterized by the accumulation of aggregated Aβ peptides in senile plaques and vascular deposits. Aβ peptides are derived from amyloid precursor protein (APP) through sequential proteolytic cleavage of APP by β- and γ-secretases generating diverse Aβ species. Aβ can aggregate to form soluble oligomeric species and insoluble fibrillar or amorphous assemblies. Some forms of the aggregated peptides are toxic to neurons.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rabbit
- Formulation
- Phosphate-buffered solution (no preservatives or carrier proteins).
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
IHC-P - Quality tested
WB - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in immunohistochemistry (IHC-P) and immunoblotting (WB).
This antibody is reactive to the C-terminus of beta amyloid and specific for the isoform that ends at the 42nd amino acid. -
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2564763 (BioLegend Cat. No. 812101)
- Disclaimer
-
Covered by US patents 5,675,063 and 7,429,487. Sold under license from Epitomics.
Antigen Details
- Structure
- Expected MW: 4 kD
- Distribution
-
Tissue distribution: Primarily nervous system, but also adipose tissue, intestine, muscle.
Cellular distribution: Cytosol, endosomes, nucleus, plasma membrane, extracellular, and golgi apparatus. - Function
- The normal function of Aβ is not well understood. Several potential physiological roles have been proposed, including: activation of kinase enzymes; protection against oxidative stress; regulation of cholesterol transport; transcription factor, and as an anti-microbial agent.
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation
- Molecular Family
- APP/β-Amyloid
- Gene ID
- 351 View all products for this Gene ID
- UniProt
- View information about beta-Amyloid x-42 on UniProt.org
Other Formats
View All β-Amyloid, x-42 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Biotin anti-β-Amyloid, x-42 | 1-11-3 | ELISA,IHC |
| Purified anti-β-Amyloid, x-42 | 1-11-3 | IHC-P,WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Biotin anti-β-Amyloid, x-42
Positive staining of Clone 1-11-3 on formalin-fixed paraffin... -
Purified anti-β-Amyloid, x-42
IHC staining of purified anti-β-Amyloid, x-42 antibody (clon...