- Clone
- 15B8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- β-catenin, CTNNB1
- Isotype
- Mouse IgG1, κ
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Western blot of purified anti-β Catenin 1 antibody (clone 15B8). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate. The blot was incubated with 0.2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-β Catenin 1 antibody (clone 15B8) on formalin-fixed paraffin-embedded mouse colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-β Catenin 1 antibody (clone 15B8) on formalin-fixed paraffin-embedded rat colon tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
Human colon adenocarcinoma cell line SW480 treated (open historgram) or untreated (filled histogram) with tankyrase inhibitor XAV939 for 18 hours, then treated with True-Nuclear™ Transcription FactorBuffer set (Cat. No. 424401) and stained with purified anti-β Catenin 1 (clone 15B8), followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 862601 | 25 µg | $106.00 | |||
| 862602 | 100 µg | $276.00 | |||
β catenin is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. β catenin also plays a key role in Wnt signaling pathways and thus is involved in neural differentiation, synaptic plasticity, neurodegenerative disease, and prevention of apoptosis. This protein also binds to adenomatous polyposis coli, which is mutated in the adenomatous polyposis of the colon.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P, ICFC - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.2 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 10 µg/mL is suggested. For intracellular flow cytometry using our True-Nuclear™ Transcription Factor Staining Protocol, the suggested use of this reagent is ≤ 0.06 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
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Application References
(PubMed link indicates BioLegend citation) -
- Guo J, et al. 2018. Cell Res. 28:1141 (IHC-P)
- Kisoh K, et al. 2017. Mol Neurobiol. 54:7917 (WB)
- Uribe RA, et al. 2015. J Cell Biol. 4:815 (IHC-F)
- Product Citations
-
- RRID
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AB_2801245 (BioLegend Cat. No. 862601)
AB_2801245 (BioLegend Cat. No. 862602)
Antigen Details
- Structure
- β Catenin is a 781 amino acid protein with an apparent molecular mass of ~ 86 kD.
- Distribution
-
Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Plasma membrane, cytoskeleton, nucleus, cytosol, and extracellular - Function
- β Catenin is a dual functional protein involved in cell-cell adhesion and gene transcription.
- Interaction
- E-cadherin/CDH1, LEF1, TCF4, etc.
- Antigen References
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- Dias C, et al. 2014. Nature. 516(7529): 51
- Rosiles A, et al. 2016. CNS Neurol Disord Drug Targets. 15(1):3
- Na K, et al. 2017. Anticancer Res. 37(6):3249
- Zhu P, et al. 2018. Cancer Res Treat. 50(4):1452
- Gene ID
- 1499 View all products for this Gene ID 12387 View all products for this Gene ID 84353 View all products for this Gene ID
- UniProt
- View information about beta Catenin 1 on UniProt.org
Other Formats
View All β Catenin 1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-β Catenin 1 | 15B8 | WB,IHC-P,ICFC |
| PE anti-β Catenin 1 | 15B8 | ICFC |
| PE/Cyanine7 anti-β Catenin 1 | 15B8 | ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-β Catenin 1
Western blot of purified anti-β Catenin 1 antibody (clone 15... 
IHC staining of purified anti-β Catenin 1 antibody (clone 15... 
IHC staining of purified anti-β Catenin 1 antibody (clone 15... 
Human colon adenocarcinoma cell line SW480 treated (open his... -
PE anti-β Catenin 1
Human colon adenocarcinoma cell line SW480 treated (open his... -
PE/Cyanine7 anti-β Catenin 1
Human colon adenocarcinoma cell line SW480 were treated with...