- Clone
- CU-18 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- EPCAM, Epithelial cell adhesion molecule, epithelial glycoprotein 314, Tumor-Associated Calcium Signal Transducer 1, Adenocarcinoma-Associated Antigen, Cell Surface Glycoprotein Trop-1, CD326 Antigen
- Isotype
- Mouse IgG1, κ
-
IHC staining of anti-BRST-1 antibody (clone CU-18) on formalin-fixed paraffin-embedded human breast tumor tissue. The tissue was incubated with the primary antibody at 1.47 µg/ml for 20 minutes at room temperature. BioLegend's Ultra Streptavidin (USA) HRP Kit (Multi-Species) was used for detection followed by counterstaining with hematoxylin.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 915004 | 100 µg | $229.00 | |||
BRST1 is a carcinoma-associated antigen and a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was generated against a protein secreted by the T47D (clone 11) human breast carcinoma cell line.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
CU-18 detects a human breast carcinoma associated glycoprotein BCA-225, a 225 kD glycoprotein secreted by the T47D breast carcinoma cell line.
-
Application References
(PubMed link indicates BioLegend citation) - RRID
-
AB_2616963 (BioLegend Cat. No. 915004)
Antigen Details
- Structure
- BRST-1 is a 35 kD protein.
- Distribution
-
Cytoplasm, extracellular, and nucleus.
- Function
- BRST-1 may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. BRST-1 plays a role in embryonic stem cells proliferation and differentiation.
- Biology Area
- Cell Adhesion, Cell Biology
- Gene ID
- 4072 View all products for this Gene ID
- UniProt
- View information about BRST-1 on UniProt.org
Other Formats
View All BRST-1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-BRST-1 | CU-18 | IHC-P |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-BRST-1
IHC staining of anti-BRST-1 antibody (clone CU-18) on formal...