Purified anti-c-Fos Antibody

Pricing & Availability
Clone
7D6-A9-D4 (See other available formats)
Regulatory Status
RUO
Other Names
Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog, G0/G1 Switch Regulatory Protein 7, Activator Protein 1, GOS7, cfos
Isotype
Mouse IgG1, κ
7D6-A9-D4_PURE_c-Fos_1_021522
ICC staining of purified anti-c-Fos (clone 7D6-A9-D4) (panel A) or mouse IgG1, κ isotype control (Cat. No. 400101) (panel B) on HeLa cells treated with PMA (100 ng/mL) for 4 hours. The cells were fixed with 4% PFA, permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 40X objective. Scale bar = 50 µm
  • 7D6-A9-D4_PURE_c-Fos_1_021522
    ICC staining of purified anti-c-Fos (clone 7D6-A9-D4) (panel A) or mouse IgG1, κ isotype control (Cat. No. 400101) (panel B) on HeLa cells treated with PMA (100 ng/mL) for 4 hours. The cells were fixed with 4% PFA, permeabilized with ice-cold methanol, and blocked with 5% FBS for 1 hour at room temperature. The cells were then stained with 5.0 µg/mL of the primary antibody, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG (Cat. No. 405326) for 1 hour at room temperature. Nuclei were counterstained with DAPI, and the image was captured with a 40X objective. Scale bar = 50 µm
  • 7D6-A9-D4_PURE_c-Fos_2_updated_031122
    HeLa cells treated (positive control, filled histogram) or untreated (negative control, open histogram) with 100 ng/mL of PMA for 4 hours were fixed and permeabilized using True-Phos™ buffer (Cat. No. 425401), and intracellularly stained with 0.125 µg/test purified anti-c-Fos (clone 7D6-A9-D4) followed by PE goat anti-mouse IgG (Cat. No. 405307).
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600751 25 µg $118.00
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600752 100 µg $293.00
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Description

The c-Fos protein, or FBJ murine viral (v-Fos) oncogene homolog, is a member of the Fos family of transcription factors. C-Fos forms heterodimers with proteins of the Jun family of transcription factors to form the AP-1 (Activator Protein-1) complex, which regulates critical cellular processes, including cell proliferation, differentiation, and survival. C-Fos has been demonstrated to play a critical role in angiogenesis, bone development, and in the progression of cancer. C-Fos is also often used as an indirect marker of neuronal activity.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human FOS protein fragments
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

ICC - Quality tested

ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5 - 10 μg/mL is recommended. For intraceullar flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

For ICC we recommend PFA-MeoH fixation/permeabilization. We do not recommend methanol-only and PFA+ Triton X-100 fixation/permeabilization due to dim signal.

 

For ICFC, we recommend fixation/permeabilization with the True-Phos™ Perm Buffer. Cytokine method was tried and failed due to no signal.

RRID
AB_2910472 (BioLegend Cat. No. 600751)
AB_2910472 (BioLegend Cat. No. 600752)

Antigen Details

Structure
c-Fos is a 330 amino acid protein with a predicted molecular weight of 41 kD.
Distribution

Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes

Function
FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal formation.
Interaction
Interacts with a variety of proteins including c-Jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
Biology Area
Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication, Cell Death, Cell Proliferation and Viability, Transcription Factors
Antigen References
  1. Dony C and Gruss P. 1987. Nature. 328:711-4.
  2. Grigoriadis AE, et al. 1994. Science. 266:443-8.
  3. Glover JN and Harrison SC. 1995. Nature. 373:257-61.
Gene ID
2353 View all products for this Gene ID
UniProt
View information about c-Fos on UniProt.org
Go To Top Version: 1    Revision Date: 02/15/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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