- Clone
- Poly6414 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Oncogene Fos, FBJ murine viral (v-fos) oncogene homolog
- Isotype
- Rabbit Polyclonal IgG
-
Total cell lysates (15 µg protein) from HT29, HCT116 and Raw264.7 treated with PMA 100ng/mL for one hour or without treatment were resolved by 4-12% Bis-tris gel electrophoresis, transferred to nitrocellulose, and probed with purified anti-c-Fos (clone poly6414). Proteins were visualized using a goat anti-rabbit IgG secondary antibody conjugated to HRP and chemiluminescence detection. Purified anti-GAPDH Antibody was used as a loading control. -
Whole cell extracts (15 µg protein) from serum-starved HeLa cells untreated (-) or treated (+) with 200 nM PMA for 4 hours were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane and probed with a 1:1000 dilution of Purified anti-c-Fos Antibody, clone Poly6414, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP anti-rabbit IgG Antibody (Cat. No. 410406) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH Antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular Weight marker.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 641401 | 25 µL | $112.00 | |||
| 641402 | 100 µL | $265.00 | |||
The c-fos protein, also known as FBJ murine viral (v-fos) oncogene homolog , is a 62 kD cellular oncogene. The c-fos protein contains a bZIP region with a DNA-binding domain and a leucine zipper domain. Together with c-jun, c-jun proteins form the heterodimeric AP-1 complex that binds to the TRE elements of cellular and viral genes including collagenase, interleukin 2, stromelysin, SV40 and polyoma among others. c-fos is expressed in a variety of normal adult tissues and has been implicated as a critical transcription factor for skeletal formation and maintenance. This transcription factor is modified by phosphorylation by a number of upstream kinases and has been shown to interact with a variety of other proteins involved in transcriptional regulation. c-fos has been implicated in the development of fibrous bone dysplasia and osteosarcoma. The c-fos protein has been shown to interact with a variety of other proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 50% glycerol.
- Preparation
- The antibody was purified by antigen-affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Upon receipt, store at -20°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1:1000 - 1:4000 antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product may contain other non-IgG subtypes.
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). -
Application References
(PubMed link indicates BioLegend citation) - Product Citations
-
- RRID
-
AB_2106632 (BioLegend Cat. No. 641401)
AB_2106632 (BioLegend Cat. No. 641402)
Antigen Details
- Structure
- c-fos heterodimerizes with c-jun to form the transcription factor AP-1; contains a bZIP region with a DNA-binding domain and a leucine zipper domain; approximately 62 kD.
- Distribution
-
Expressed in the nucleus of many tissues/cells including placenta, brain, thyroid, central nervous system, lung, endometrium, and B lymphocytes.
- Function
- FOS and JUN oncoproteins form dimeric complexes that stimulate transcription of genes containing AP-1 regulatory elements. FOS was the first transcription factor identified to have a critical function in the development of cells required for skeletal form
- Interaction
- Interacts with a variety of proteins including c-jun, TFIID, NCOA2, NFAT1, CHOP, ELK1, SRC1, and ETS1
- Modification
- Phosphorylation on multiple sites (threonine, serine) by MAP kinases, ERK2, EphB2, PKA, Rsk.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Neuroscience, Synaptic Biology, Transcription Factors
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Dony C, et al. 1987. Nature 328:711.
2. Grigoriadis AE, et al. 1994. Science 266:443.
3. Glover JNM, et al. 1995. Nature 373:257. - Regulation
- c-fos levels are highly expressed in human term fetal tissues as compared to normal human tissues. c-fos levels have been reported to be increased in patients with fibrous bone dysplasia.
- Gene ID
- 2353 View all products for this Gene ID
- UniProt
- View information about c-Fos on UniProt.org
Other Formats
View All c-Fos Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-c-Fos | Poly6414 | WB |
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