- Clone
- W20054B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
- Isotype
- Rat IgG2a, κ
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Total cell lysates (15 µg total protein) from Jurkat (Lane 1, untreated control) and Jurkat treated with 1 µM of staurosporine (Lane 2) for 3 hours were resolved by 4-12 % Bis-Tris gel electrophoresis, transferred to a nitrocellulose membrane, and probed with 0.5 μg/mL of anti-Caspase-3 antibody overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-rat IgG antibody (Cat. No. 405405) at 1:3000 dilution. Direct-Blot™ HRP anti-β actin antibody (Cat. No. 643807) was used as a loading control at a 1:10,000 dilution (lower). Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection reagent. Lane M: Molecular weight ladder
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 949801 | 25 µg | $118.00 | |||
| 949802 | 100 µg | $293.00 | |||
Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8, and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Partial recombinant human Caspase-3 protein
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody recognizes endogenous human Caspase-3 proteins in both total and spliced forms from Jurkat cell lysates treated with or without staurosporine.
- RRID
-
AB_2904464 (BioLegend Cat. No. 949801)
AB_2904464 (BioLegend Cat. No. 949802)
Antigen Details
- Structure
- Caspase 3 is a 277 amino acid protein with a molecular mass of 32 kD.
- Distribution
-
Nucleus and cytosol
- Function
- Caspase 3 regulates programmed cell death (apoptosis)
- Interaction
- Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo
- Cell Type
- B cells, Endothelial cells, Fibroblasts, Monocytes, T cells
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Cell Death, Neuroscience
- Antigen References
-
- Goldberg YP, et al. 1996. Nat Genet. 13:442-9.
- Fernandes-Alnemri T, et al. 1996. Proc Natl Acad Sci U S A. 93:7464-9.
- Slee EA, et al. 2001. J Biol Chem. 276:7320-6.
- Woo M, et al. 2003. Nat Immunol. 4:1016-22.
- Gene ID
- 836 View all products for this Gene ID
- UniProt
- View information about Caspase-3 on UniProt.org
Other Formats
View All Caspase-3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Caspase-3 | W20054B | WB |
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Purified anti-Caspase-3
Total cell lysates (15 µg total protein) from Jurkat (Lane 1...