- Clone
- Poly6341 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Caspase 3 preproprotein, Casp3, cpp32, Apopain, Yama, PARP cleavage protease, SREBP cleavage activity 1
- Isotype
- Rabbit Polyclonal IgG
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Western blot analysis of extracts from Hela cells, using anti-caspase-3, Poly6341.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 634101 | 125 µL | $112.00 | |||
Caspase 3 is a member of the peptidase family C14. This cytoplasmic caspase functions as a heterotetramer and has two isoforms (one acts as a dominant negative inhibitor). The pro-form of this caspase is approximately 32 kD. Caspase 3 is involved in the caspase activation cascade responsible for apoptosis execution. Caspase 3 is inhibited by c-IAP1, c-IAP2, XIAP, survivin, and Livin and activated by Caspase 6, -8 , and -10, granzyme B, Apaf-1 by cleavage into large p17 and small p12 subunits. Caspase 3 has been shown to cleave PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topoisomerase I, vimentin, Rb, lamin B, mdm2, and β-catenin. The poly6341 antibody has been shown to be useful for western blotting, immunoprecipitation and immunohistochemistry of human Human caspase-3 precursor.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Peptide mapping to the amino terminal domain of human caspase-3 prescursor
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide and 0.2% gelatin.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration and expiration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C. DO NOT FREEZE.
- Application
-
WB - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. Western blotting suggested working dilution(s): Use 25µl per 5 ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product may contain other non-IgG subtypes. - Additional Product Notes
- The 125 µL size can be used for approximately 5 Western blots.
- Product Citations
-
- RRID
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SCR_001134 (BioLegend Cat. No. 634101)
Antigen Details
- Structure
- Peptidase family C14, heterotetramer. Two isoforms, pro-Casp3 32 kD
- Interaction
- Active heterodimers between large p17 subunit and small p11 subunit of Casp7 and vice versa. Cleaves PARP, huntingtin, androgen receptor, atrophin-1, DNA- PKcs, D4-GDI, hnRNPs C1/C2, U1snRNP, PKC delta, ATM, fodrin, gelsolin, ICAD, RIP, X-IAP, STAT1, topo
- Bioactivity
- Involved in caspase activation cascade responsible for apoptosis execution. Cleaves/activates SREBPs, Casp6, Casp7, Casp9
- Biology Area
- Apoptosis/Tumor Suppressors/Cell Death, Cell Biology, Neuroscience
- Antigen References
-
1. Goldberg Y, et al. 1996. Nat. Genet. 13:442.
2. Fernandes-Alnemri T, et al. 1996. P. Natl. Acad. Sci. USA 93:7464.
3. Slee E, et al. 2001. J. Biol. Chem. 276:7320.
4. Woo M, et al. 2003. Nat. Immunol. 4:1016. - Regulation
- Inhibited by dominant negative isoform, c-IAP1, c-IAP2, XIAP, survivin, Livin. Activated when cleaved by Casp6, -8, -10, granzyme B, Apaf-1 into large p17 and small p12 subunits
- Gene ID
- 12367 View all products for this Gene ID
- UniProt
- View information about Caspase-3 on UniProt.org
Other Formats
View All Caspase-3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Caspase-3 | Poly6341 | WB,IP |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Caspase-3
Western blot analysis of extracts from Hela cells, using ant...