- Clone
- A22001B (See other available formats)
- Regulatory Status
- RUO
- Other Names
- KIT, Mast/Stem Cell growth factor receptor Kit, CD117, C-KIT, PBT, Tyrosine-Protein Kinase Kit, SCFR, V-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog
- Isotype
- Mouse IgG1, κ
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Whole cell extracts (15 µg total protein) from HEL cells untreated or treated with HSCF (50 ng/mL, 5 minutes), were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL of purified anti-CD117 Phospho (Tyr703) (clone A22001B) (left panel) or anti-CD117 Pan (right panel) for 2 hours. Proteins were visualized by chemiluminescence detection using HRP Goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 619551 | 25 µg | $176.00 | |||
| 619552 | 100 µg | $440.00 | |||
Proto-oncogene CD117/c-KIT is a type III receptor tyrosine kinase that binds Stem Cell Factor (SCF). CD117 is used to identify certain types of hematopoietic progenitors in bone marrow, such as hematopoietic stem cells (HSC), multipotent progenitors (MPP), and common myeloid progenitors (CMP). Expression of CD117/c-KIT is also found in stem cell precursors from prostate, liver, and heart. CD117/c-KIT signaling plays a role in cell survival, proliferation, and differentiation; hence, its activation is required for melanocyte survival, hematopoiesis and gametogenesis. Aberrant activation of CD117/c-KIT due to gene mutations has been associated with gastrointestinal stromal tumors, testicular seminoma, mast cell disease, melanoma, acute myeloid leukemia, and others, while loss of activation is associated with piebaldism.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide from human CD117 (c-KIT) phosphorylated at Tyr703.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.25 - 1.0 µg/mL. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_3083404 (BioLegend Cat. No. 619551)
AB_3083404 (BioLegend Cat. No. 619552)
Antigen Details
- Structure
- c-KIT is a 976 amino acid protein with a predicted molecular weight of 110 kD. It is a type III receptor tyrosine kinase protein that is composed of five immunoglobulin-like domains on the extracellular side and an intracellular tyrosine kinase domain.
- Distribution
-
c-KIT is a transmembrane protein but can also be found in cytoplasmic vesicles after internalization.
- Function
- Regulation of cell survival and proliferation, Hematopoiesis, Stem cell maintenance, Gametogenesis, Mast cell development, Melanogenesis
- Interaction
- Activate AKT1 via phosphorylation of PIK3R1, activate RAS, RAF1, and MAP kinases, promote activation of STAT family members
- Ligand/Receptor
- c-KIT is the receptor for Mast/Stem cell growth factor (SCF)
- Biology Area
- Cancer Biomarkers, Cell Biology, Immuno-Oncology
- Molecular Family
- CD Molecules, Phospho-Proteins
- Antigen References
-
- Myburgh R, et al. 2020. Leukemia. 34:2688-703.
- Miettinen M, et al. 2005. Appl Immunohistochem Mol Morphol. 13:205-20.
- Cananzi M, et al. 2012. Organogenesis. 8:77-88.
- Harris KS, et al. 2021. Sci Rep. 11:1465.
- Gene ID
- 3815 View all products for this Gene ID
- UniProt
- View information about CD117 on UniProt.org
Other Formats
View All CD117 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-CD117 (c-KIT) Phospho (Tyr703) | A22001B | WB |
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Purified anti-CD117 (c-KIT) Phospho (Tyr703)
Whole cell extracts (15 µg total protein) from HEL cel...