- Clone
- BC3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- T-cell surface glycoprotein CD3 epsilon chain, CD3-epsilon, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e antigen, epsilon polypeptide (TiT3 complex), T-cell antigen receptor complex, epsilon subunit of T3, T3E, TCRE
- Previously
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Covance Catalog# MMS-5212
- Isotype
- Mouse IgG2b, κ
-
Human peripheral blood lymphocytes were stained with purified anti-CD3 antibody (clone BC3, green histogram) or purified mouse IgG2b, κ isotype control (orange histogram), followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 830301 | 100 µL | $275.00 | |||
CD3ε is a 20 kD chain of the CD3/T-cell receptor (TCR) complex which is composed of two CD3ε, one CD3γ, one CD3δ, one CD3ζ (CD247), and a T-cell receptor (α/β or γ/δ) heterodimer. It is found on all mature T lymphocytes, NK-T cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against human CD3.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is 1.0 - 2.0 µg/ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody is effective in flow cytometry (FC).
- RRID
-
AB_2564937 (BioLegend Cat. No. 830301)
Antigen Details
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- CD Molecules
- Antigen References
-
1. Barclay N, et al. 1993. The Leucocyte FactsBook. Academic Press. San Diego.
2. Beverly P, et al. 1981. Eur. J. Immunol. 11:329.
3. Lanier L, et al. 1986. J. Immunol. 137:2501-2507. - Gene ID
- 916 View all products for this Gene ID
- UniProt
- View information about CD3 on UniProt.org
Other Formats
View All CD3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-CD3 | BC3 | FC |
| TotalSeq™-A1455 anti-CD3 | BC3 | PG |
| TotalSeq™-C1455 anti-CD3 | BC3 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-CD3
Human peripheral blood lymphocytes were stained with purifie... -
TotalSeq™-A1455 anti-CD3
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TotalSeq™-C1455 anti-CD3