- Clone
- 9A6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- carcinoembryonic antigen-related cell adhesion molecule 6
- Previously
-
Signet Catalog# 750-001
Signet Catalog# 750-005
Covance Catalog# SIG-3750
- Isotype
- Mouse IgG1, κ
-
IHC staining of purified anti-CD66C (CEACAM 6) antibody (clone 9A6) on formalin-fixed paraffin-embedded human colon tissue. The tissue was incubated with a 1:40 dilution of the primary antibody for 20 minutes at room temperature. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. -
Immunofluorescence of THP-1 cells with (A) mouse IgG1, κ isotype control (Negative, Cat. No. 401402) or (B-D) CD66C primary antibody (Clone 9A6). Alexa Fluor® 488 (Green) Goat anti-Mouse IgG (Cat. No. 405319) was used as secondary antibody. Nuclei were counterstained with DAPI (Blue, Cat. No. 422801). The image was captured with a 60X objective using KEYENCE BZ-X700 fluorescence microscope. Exposure time (Seconds) for (A) is 1/12, and (B-D) is 1/15. Concentrations for (A, B) is 1/100, (C) is 1/200 and (D) is 1:500.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 908601 | 100 µL | $253.00 | |||
Anti-human CEACAM6 (CEA related cell adhesion molecule 6, non-specific cross reacting antigen (NCA); CD66c)
CEACAM6 is a member of the carcinoembryonic antigen (CEA) gene family. Like other members of the CEA family, CEACAM6, consists of a single N domain, with structural homology to the immunoglobulin variable domains, followed by one immunoglobulin constant like A and B domain.
CEACAM6 encodes a glycosyl phosphatidyl inositol (GPI) linked glycoprotein, which has a molecular weight of 90kDa.
CEACAM6 expression is upregulated in many adenocarcinomas and leukemias. CEACAM6 is expressed in epithelial cells of the fetal and adult gastrointestinal tract, epithelia of glandular tissues, squamous epithelial cell of the tongue, esophagus and cervix as well as on granulocytes.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This antibody was developed against a tumor cell line expressing CEACAM6.
- Formulation
-
Phosphate-buffered solution with BSA + 0.09% NaN3.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Certificate of Analysis online tool.)
- Storage & Handling
- Store between 2°C and 8°C.
- Application
-
IHC-P - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by formalin-fixed paraffin-embedded immunohistochemical staining. For immunohistochemistry, a dilution of 1:40 is suggested. For immunocytochemistry, a concentration range of 1:100 - 1:200 dilution is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
-
Application References
(PubMed link indicates BioLegend citation) -
- Hammarstrom S. 1999. Semin Cancer Biol 9:67-81. (EM)
- Hanenberg H, et al. 1994. Leukemia. 12:2127. (ICC)
- Daniel S, et al. 1993. Int J Cancer. 55:303. (IHC)
- Gottlinger HG, et al. 1988. Cancer Res. 48:2198. (ICC)
- RRID
-
AB_2565088 (BioLegend Cat. No. 908601)
Antigen Details
- Structure
- Expected MW: 45 kD
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- 4680 View all products for this Gene ID
- UniProt
- View information about CD66C on UniProt.org
Other Formats
View All CD66C (CEACAM 6) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-CD66C (CEACAM 6) | 9A6 | IHC-P,ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-CD66C (CEACAM 6)
IHC staining of purified anti-CD66C (CEACAM 6) antibody (clo... 
Immunofluorescence of THP-1 cells with (A) mouse IgG1, κ iso...