- Clone
- POH-1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cell division cycle 2, G1 to S and G2 to M, cyclin dependent kinase 1, p34 kinase, CDK1
- Isotype
- Mouse IgG2a, κ
-
Total cell lysates (15 µg protein) from A431 (lane 1), A431 (lane 2), A431 (lane 3), Molt-4 (lane 4) and HeLa (lane 5) cells were resolved by electrophoresis (4-12% Bis-Tris gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution) (lane 1), 1 µg/mL (1:500 dilution) (lane 2) and 2 µg/mL (1:250 dilution) (lane 3, 4, 5) Purified anti-cdc2 (p34) antibody, clone POH-1 (upper panel). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated with HRP for anti-cdc2 (p34) antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1 (lower panel). Lane M: molecular weight ladder. -
Hela cells stained with purified mouse monoclonal antibody against Cdc2(p34) (clone POH-1), followed by Rhodamine Red-X conjugated goat anti-mouse IgG and DAPI
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 626901 | 25 µg | $101.00 | |||
Cdc2 (p34), also known as cell division cycle 2, G1 to S and G2 to M; cyclin dependent kinase 1, p34 kinase, and CDK1, is serine/threonine kinase that is ubiquitously expressed in eukaryotes. Cdc2 is found as both a cytoplasmic and nuclear protein. cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis universally in eukaryotes. The cdc2 protein is highly phosphorylated on threonine, serine, and tyrosine; tyrosine phosphorylation is subject to cell cycle regulation. Cdc2 has been shown to interact with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, and p53. The POH-1 antibody recognizes human, monkey and bovine cdc2 and is useful for Western blotting. The POH-1 antibody has also been reported to be useful for immunoprecipitation, immunohistochemistry (formalin-fixed, paraffin embedded), and immunofluorescence.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human cdc2 protein
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.4, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- This antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified
IP, IHC - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone does not react with Mouse, Rat, Xenopus, Drosophila.
Additional reported applications (for the relevant formats) include: immunohistochemistry1 of formalin-fixed, paraffin-embeded tissue sections and immunoprecipitation. -
Application References
(PubMed link indicates BioLegend citation) -
- Lukas J, et al. 1992. Eur. J. Biochem. 207:169.
- Product Citations
-
- RRID
-
AB_2074779 (BioLegend Cat. No. 626901)
Antigen Details
- Structure
- Serine/threonine kinase; molecular weight approximately 34 kD
- Distribution
-
Ubiquitous expression in eukaryotes, cytoplasmic and nuclear.
- Function
- cdc2 is a catalytic subunit of a protein kinase called the M-phase promoting factor that induces entry into mitosis universally among eukaryotes.
- Interaction
- Interacts with numerous proteins including cyclin A1, cyclin A2, cyclin B1, cyclin B2, BRCA1, dynamin 2, Fyn, Lyn, p53
- Modification
- Highly phosphorylated (threonine, serine, tyrosine); tyrosine phosphorylation subject to cell cycle regulation
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Draetta G, et al. 1988. Nature 336:738.
- Gene ID
- 983 View all products for this Gene ID
- UniProt
- View information about cdc2 p34 on UniProt.org
Other Formats
View All cdc2 (p34) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-cdc2 (p34) | POH-1 | WB,ICC,IHC,IP |
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