- Clone
- A21009D (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cyclin Dependent Kinase 1, Cdk1, CDC28A, P34CDC2, P34 Protein Kinase
- Isotype
- Mouse IgG1, κ
-
Whole cell extracts (15 µg total protein) from HeLa cells treated with 4 mM hydroxyurea for 20 hours (+) and untreated HeLa cells (-) were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with purified anti-CDC2 Phospho (Tyr15) (clone A21009D) for 2 hours at room temperature. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG (Cat. No. 405306) at a 1:3000 dilution. Equal protein loading was confirmed using a pan CDC2 (clone POH-1) (Cat. No. 626901) and Direct-Blot™ HRP anti-GAPDH (Cat. No. 607904) was used as a loading control at a 1:50000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Lane M: Molecular weight marker -
HeLa cells treated with hydroxyurea (4 mM, 20 hours) (positive control, filled histogram) and untreated HeLa cells (negative control, open histogram) were fixed with ice-cold methanol and permeabilized using the True-Phos™ Perm Buffer (Cat. No. 425401) and intracellularly stained with purified anti-CDC2 Phospho (Tyr15) (clone A21009D) or purified Mouse IgG1, κ isotype control (Cat. No. 400102) (open histogram, dashed line) followed by PE goat anti-mouse IgG (Cat. No. 405307).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 613851 | 25 µg | $125.00 | |||
| 613852 | 100 µg | $335.00 | |||
Cell division control protein 2 (CDC2) also known as Cyclin-dependent kinase 1 (CDK1) is a key regulatory kinase essential for cell growth, proliferation, cell cycle progression, and early embryonic development. It is responsible for driving cells through G2 phase into M phase. Prior to mitosis, CDC2, along with its binding partner cyclin B1, exists in an inactive state when phosphorylated on Thr14 and Tyr15 by PKMYT1 (MYT1) and WEE1. This B1-CDC2 complex is activated during G2 and early mitosis by CDC2-mediated dephosphorylation. During mitosis, CDC2 inhibits DNA re-replication and subsequent polyploidy and cell senescence through the sequestering of cyclin A2 and suppression of the formation of CDK2-cyclin A complexes. CDC2 can function as a substitute for other CDC family members and drive the progression of the cell cycle in the event of their loss. CDC2 functions as a positive regulator of global translation, protein synthesis, and genome stability and participates in DNA damage repair, checkpoint activation, and DNA replication fork progression. It is overexpressed in several cancers including liver, breast, and colorectal cancers and has been implicated in the promotion of tumorigenesis.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide corresponding to human CDC2 phosphorylated at tyrosine 15
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICFC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.125 - 1.0 µg/mL. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2941613 (BioLegend Cat. No. 613851)
AB_2941613 (BioLegend Cat. No. 613852)
Antigen Details
- Structure
- CDC2 is a 297 amino acid protein with a predicted molecular weight of 34 kD.
- Distribution
-
Ubiquitously expressed/ Mitochondria, nucleus, cytoskeleton, cytoplasm
- Function
- Serine/threonine-protein kinase/ Cell Cycle
- Biology Area
- Cancer Biomarkers, Cell Biology, Cell Cycle/DNA Replication
- Molecular Family
- Phospho-Proteins
- Antigen References
-
- Chow J, et al. 2010. Mol Cell Biol. 31:1478-91.
- Diril M, et al. 2012. PNAS. 109:3826-31.
- Haneke K, et al. 2020. J Cell Biol. 219:e201906147.
- Liao H, et al. 2017. Oncotarget. 8:90662-90673.
- Prevo R, et al. 2018. Cell Cycle. 17:1513-1523.
- Gene ID
- 983 View all products for this Gene ID
- UniProt
- View information about CDC2 on UniProt.org
Other Formats
View All CDC2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-CDC2 Phospho (Tyr15) | A21009D | WB,ICFC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
-
Purified anti-CDC2 Phospho (Tyr15)
Whole cell extracts (15 µg total protein) from HeLa cells tr... 
HeLa cells treated with hydroxyurea (4 mM, 20 hours) (positi...