Purified anti-CKMT1 Antibody

Pricing & Availability
Clone
1A6-C7-G10 (See other available formats)
Regulatory Status
RUO
Other Names
CKMT1A, CKMT1B, Creatine Kinase, Mitochondrial 1A
Isotype
Mouse IgG1, κ
1A6-C7-G10_PURE_CKMT1_Antibody_1_062619
Western blot of purified anti-CKMT1 antibody (clone 1A6-C7-G10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 10 ng of recombinant human CKMT2 protein; Lane 6: 10 µg of CKMT1A overexpression lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1A6-C7-G10_PURE_CKMT1_Antibody_1_062619
    Western blot of purified anti-CKMT1 antibody (clone 1A6-C7-G10). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain lysate; Lane 3: 20 µg of mouse brain lysate; Lane 4: 20 µg of rat brain lysate; Lane 5: 10 ng of recombinant human CKMT2 protein; Lane 6: 10 µg of CKMT1A overexpression lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 1A6-C7-G10_PURE_CKMT1_Antibody_2_062619
    IHC staining of purified anti-CKMT1 antibody (clone 1A6-C7-G10) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 1A6-C7-G10_PURE_CKMT1_Antibody_3_062619
    IHC staining of purified anti-CKMT1 antibody (clone 1A6-C7-G10) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 1A6-C7-G10_PURE_CKMT1_Antibody_4_062619
    IHC staining of purified anti-CKMT1 antibody (clone 1A6-C7-G10) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R. (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend's Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
  • 1A6-C7-G10_PURE_CKMT1_Antibody_5_022020
    ICC staining of purified anti-CKMT1 antibody (clone 1A6-C7-G10) on SH-SY5Y Cells. The cells were fixed with 4% PFA, permeabilized with a buffer containing 0.1% Triton X-100 and 0.25% BSA, and blocked with 2% normal goat serum and 0.02% BSA. The cells were then incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with 2.5 µg/mL of Alexa Fluor® 594 goat anti-mouse IgG for one hour at room temperature. The cells were co-stained with Flash Phalloidin™ Green 488 (Cat. No. 424201). The slide was mounted with fluoromount G with DAPI. The image was captured with a 60X objective. Scale bar: 20 µm
Cat # Size Price Quantity Check Availability
867201 25 µg $106.00
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867202 100 µg $276.00
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Description

CKMT1 is the mitochondrial specific creatine kinase subunit that is ubiquitously expressed in mammals. CKMT1 is mainly localized to the mitochondrial intermembrane space and plays an important role in cellular energetics. Impairment in CKMT1 due to oxidative and radical-induced damage has been reported in numerous diseases including neurodegenerative disorders. Overexpression of CKMT1 is observed in several tumors in which high energy consumption is required for proliferation.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Purified recombinant human CKMT1 fragment expressed in E. coli.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.0 - 10 µg/mL is suggested. For immunocytochemistry, a concentration range of 2.0 - 10 μg/mL is recommended. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Fihurka O, et al. 2023. Biomolecules. 13:. PubMed
  2. Fihurka O, et al. 2022. Int J Mol Sci. 23:. PubMed
RRID
AB_2810799 (BioLegend Cat. No. 867201)
AB_2810799 (BioLegend Cat. No. 867202)

Antigen Details

Structure
CKMT1 is a 417 amino acid protein with predicted and observed molecular mass of ~47 kD.
Distribution

Tissue Distribution: Mainly expressed in brain, appendix, tonsil, skin, and colon
Cellular Distribution: Mitochondrion and cytosol

Function
CKMT1 plays a central role in energy transduction in tissues with large, fluctuating energy demands such as the brain.
Interaction
CKMT1 exists as an octamer composed of four homodimers
Cell Type
Neurons
Biology Area
Cancer Biomarkers, Cell Death, Neurodegeneration, Neuroinflammation, Neuroscience
Molecular Family
Mitochondrial Markers
Antigen References
  1. Gramage E, et al. 2013. Toxicology. 10.1016/j.tox.2013.02.013.
  2. Tanimura A, et al. 2014. PLoS One. 9(2):e89916.
  3. Onda T, et al. 2006. Br J Cancer. 94:698-709.
Gene ID
1159 View all products for this Gene ID 12716 View all products for this Gene ID 29593 View all products for this Gene ID
UniProt
View information about CKMT1 on UniProt.org
Go To Top Version: 2    Revision Date: 02/20/2020

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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