- Clone
- BL28583 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Cystatin B, CSTB, Stefin B, CST6, STFB
- Isotype
- Mouse IgG2b, κ
-
Western blot of purified anti-CSTB antibody (BL28583). Lane 1: Molecular weight marker; Lane 2: 20 µg of rat brain lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 858301 | 25 µg | $106.00 | |||
Cystatin B (CSTB) is a type 1 cystatin belonging to the cystatin superfamily. There are three cystatin subfamilies including type 1 cystatins, type 2 cystatins, and kininogens. Cystatins are thought to have a protective role against the proteases that leak from the lysosomes by inhibiting their cysteine proteinase activity. CSTB can covalently form dimers, tetramers, or oligomers. CSTB oligomers bind to aggregated amyloid-beta (Aβ) and reduce Aβ fibril formation.
Product Details
- Verified Reactivity
- Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant rat CSTB (amino acid sequence Met2~Phe98)
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2814580 (BioLegend Cat. No. 858301)
Antigen Details
- Structure
- CSTB is a 98 amino acid protein with predicted molecular mass of ~11 kD and observed molecular mass of ~14 kD.
- Distribution
-
Tissue Distribution: Predominantly expressed in the tonsil, oral mucosa, and bladder
Cellular Distribution: Cytosol, nucleus, and extracellular - Function
- CSTB protects cells from oxidative stress and injury from lysosomal protease leakage.
- Interaction
- CSTB is an inhibitor of cysteine proteinases such as papain and cathepsin B, cathepsin H, and cathepsin L.
- Cell Type
- Microglia, Neurons
- Biology Area
- Neurodegeneration, Neuroscience, Protein Trafficking and Clearance
- Molecular Family
- APP/Aβ Degradation, Enzymes and Regulators, Proteases
- Antigen References
-
- Skerget K, et al. 2010. J Biol Chem. 29:285.
- Tegelberg S, et al. 2012. J Neuropathol Exp Neurol. 71:40.
- Žerovnik E, et al. 2019. Cells. 18:8.
- Gene ID
- 25308 View all products for this Gene ID
- UniProt
- View information about CSTB on UniProt.org
Other Formats
View All CSTB Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-CSTB | BL28583 | WB |
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Purified anti-CSTB
Western blot of purified anti-CSTB antibody (BL28583). Lane ...