- Clone
- 10B1 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- DNA-protein kinase catalytic subunit, DNA-activated protein kinase, DNA-dependent protein kinase
- Isotype
- Mouse IgG1, κ
-
3 minutes UV treated HeLa cells (recovered 30 minute) were fixed with 4% paraformaldehyde (PFA) and then were permeabilized with 0.5% Triton X-100 followed by blocking with 5% FBS. The cells were then intracellularly stained with 5 µg/ml anti-DNA-PKcs phospho (Thr2609) (clone 10B1) antibody in blocking buffer overnight at 4°C followed by staining with Alexa Fluor® 488 conjugated goat anti-mouse IgG antibody (clone Poly4053) for one hour at room temperature. The images were captured with a 40X objective. -
Untreated HeLa cells were fixed with 4% paraformaldehyde (PFA) and then were permeabilized with 0.5% Triton X-100 followed by blocking with 5% FBS. The cells were then intracellularly stained with 5 µg/ml anti-DNA-PKcs phospho (Thr2609) (clone 10B1) antibody in blocking buffer overnight at 4°C followed by staining with Alexa Fluor® 488 conjugated goat anti-mouse IgG antibody (clone Poly4053) for one hour at room temperature. The images were captured with a 40X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 612901 | 25 µg | $118.00 | |||
| 612902 | 100 µg | $293.00 | |||
DNA-protein kinase catalytic subunit (DNA-PKcs) also known as DNA-activated kinase is a nuclear 460-470 kD serine threonine kinase involved in double-stranded DNA break repair, VDJ recombination, and transcriptional modulation. DNA-PKcs must bind DNA ends to become active. DNA-PKcs is modified by phosphorylation and has been shown to interact with Ku70/Ku80, KIP, DNA-ligase IV, and XRCC4 proteins. Phosphorylated DNA-PKcs is upregulated after DNA damaage. The 10B1 monoclonal antibody recognizes phosphorylated human DNA-PKcs (Thr2609) and has been shown to be useful for immunofluorescence staining and Western blotting.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Modified peptide
- Formulation
- This DNA-PKcs antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide. Final antibody concentration is 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
ICC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunocytochemistry. For immunocytochemistry, a concentration range of 5.0 - 10 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_315784 (BioLegend Cat. No. 612901)
AB_315784 (BioLegend Cat. No. 612902)
Antigen Details
- Structure
- PI3/PI4-Kinases family. Spliced products 460 kD, 470 kD
- Distribution
-
Nuclear
- Function
- Serine/Threonine kinase, dsDNA break repair, VDJ recombination, transcriptional modulator
- Interaction
- Ku70/Ku80, KIP, DNA-ligase IV, XRCC4
- Modification
- Phosphorylation
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Signal Transduction, Transcription Factors
- Molecular Family
- Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
1. Hartley K, et al. 1995. Cell. 82:849.
2. Connelly M, et al. 1996. Gene. 175:271.
3. Douglas P, et al. 2002. Biochem. J. 368:243
4. Calsou P, et al. 2003. J. Mol. Biol. 326:93. - Regulation
- Must bind DNA ends to become active
- Gene ID
- 5591 View all products for this Gene ID
- UniProt
- View information about DNA-PKcs Phosphorylated on UniProt.org
Other Formats
View All DNA-PKcs Phospho (Thr2609) Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-DNA-PKcs Phospho (Thr2609) | 10B1 | ICC |
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Purified anti-DNA-PKcs Phospho (Thr2609)
3 minutes UV treated HeLa cells (recovered 30 minute) were f... 
Untreated HeLa cells were fixed with 4% paraformaldehyde (PF...