- Clone
- 17C11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Eukaryotic translation initiation factor 3 subunit I, Eukaryotic translation initiation factor 3 subunit 2, eIF-3-beta, eIF3 p36, TGF-beta receptor-interacting protein 1, TRIP-1
- Isotype
- Mouse IgG2a, κ
-
Total lysates (15 µg protein) from Ramos (Lane 1), HeLa (Lane 2) and NIH/3T3 (Lane 3) cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL purified anti-eIF3I (p36) antibody, clone 17C11 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for the anti-eIF3I (p36) antibody or 1:5000 diluted Direct-Blot™ HRP anti-β-Actin antibody, clone 2F1-1 (lower). Lane M: Molecular weight ladder
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 646701 | 25 µg | $124.00 | |||
| 646702 | 100 µg | $265.00 | |||
The elF3I (eIF3-p36) is a subunit of eukaryotic translation initiation factor 3 (eIF3) complex that plays a central role in the protein translation initiation. The eIF3 consists of at least 10 subunits ranging from 35 to 170 kd. elF3-p36 is constitutively expressed in many tissues, including spleen thymus, prostate, colon, ovary, etc.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.25 to 0.5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_1732022 (BioLegend Cat. No. 646701)
AB_1732022 (BioLegend Cat. No. 646702)
Antigen Details
- Distribution
-
Expressed in most tissues, including spleen, thymus, prostate, ovary, etc.
- Function
- Translation initiation.
- Biology Area
- Cell Biology, Protein Synthesis
- Molecular Family
- Nuclear Markers
- Antigen References
-
1. Tymms MJ. et al. 1997. Oncogene 15:2449
2. H TK. et al. 2000 Blood 96:747 - Gene ID
- 8668 View all products for this Gene ID
- UniProt
- View information about eIF3I on UniProt.org
Other Formats
View All eIF3I Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-eIF3I (p36) | 17C11 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-eIF3I (p36)
Total lysates (15 µg protein) from Ramos (Lane 1), HeLa (Lan...