- Clone
- 10C4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- RNA polymerase II transcription factor SIII p18 subunit, transcription elongation factor B polypeptide 2, TCEB2
- Isotype
- Mouse IgG1, κ
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Total cell lysates (15 µg protein) from HeLa cells were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL (1:1000 dilution, lane 1), 1 µg/mL (1:500 dilution, lane 2) and 2 µg/mL (1:250 dilution, lane 3) purified anti-Elongin B antibody, clone 10C4 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-Elongin B antibody or 1:5000 diluted Direct-Blot HRP anti-β-Actin antibody, clone 2F1-1(lower). Lane M: Molecular weight ladder.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 629302 | 100 µg | $229.00 | |||
Elongin B (also known as RNA polymerase II transcription factor SIII p18 subunit and transcription elongation factor B polypeptide 2) is a member of the UbH family containing a ubiquitin-like domain. Elongin functions as a subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites in the nucleus. The Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases. Clone 10C4 recognizes the human elongin B protein and is useful for Western blotting.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Full length recombinant protein
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide at 0.5 mg/ml.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2201267 (BioLegend Cat. No. 629302)
Antigen Details
- Structure
- UbH family, ubiquitin-like domain, heterotrimer; Human Elongin B has two isoforms with predicted MW of 13kD and 18kD
- Distribution
-
Nuclear
- Function
- Regulatory subunit of a general transcription elongation factor that increases RNA polymerase II transcription elongation past template-encoded arresting sites
- Interaction
- Elongin BC complex acts as adaptor to link Elongin A, VHL, WSB1 or SOCS1 with a module of CUL2 or CUL5 and RBX1 to form E3 ubiquitin ligases
- Biology Area
- Cell Biology, DNA Repair/Replication, Transcription Factors, Ubiquitin/Protein Degradation
- Antigen References
-
1. Duan D, et al. 1995. Science. 269:1402.
2. Stebbins C, et al. 1999. Science. 284:455.
3. Brower C, et al. 2002. P. Natl. Acad. Sci. USA 99:10353.
4. DeRenzo C, et al. 2003. Nature. 424:685. - Gene ID
- 6923 View all products for this Gene ID
- UniProt
- View information about Elongin B on UniProt.org
Other Formats
View All Elongin B Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Elongin B | 10C4 | WB,IP |
Compare Data Across All Formats
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Purified anti-Elongin B
Total cell lysates (15 µg protein) from HeLa cells were reso...