- Clone
- 4B11B69 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- P44/42 MAP (motogen activated protein) Kinase, Extracelluar signal-regulated kinase (ERK)
- Isotype
- Mouse IgG2b, κ
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Total cell lysates (15 µg protein) from Jurkat cells without treatment (lane 1) and Jurkat cells starved overnight and then treated with PMA (lane 2) were resolved by electrophoresis (4-20% Tris-Glycine gel), transferred to nitrocellulose, and probed with 0.5 µg/mL Purified anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody (1:1000 dilution), clone 4B11B69 (upper). Proteins were visualized by chemiluminescence detection using a 1:3000 diluted goat anti-mouse-IgG secondary antibody conjugated to HRP for anti-ERK1/2 Phospho (Thr202/Tyr204) Antibody or a donkey anti-rabbit IgG Antibody conjugated to HRP for anti-GAPDH Antibody (lower). Lane M: Molecular weight ladder. -
Untreated (left) and PMA-treated (20 nM PMA treatment for 10 minutes, right) mouse NIH-3T3 cells were stained with purified anti-ERK1/2 Phospho (Thr202/Tyr204) (clone 4B11B69) antibody, followed by staining with DyLight™ 488 conjugated goat anti-mouse IgG (green, Cat. No. 405310) antibody and Alexa Fluor® 594 conjugated phalloidin (red). Nuclei were stained with DAPI (blue).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 675502 | 100 µg | $293.00 | |||
ERK1/2 are members of mitogen-activated kinases (MAPKs) of serine/threonine protein kinases. ERK1/2 can be activated by a range of extracellular stimuli, such as mitogen, growth factors, neurotransmitters, chemokines, and cytokines, through receptor tyrosine kinases (RTK), G protein-coupled receptors (GPCRs), or protein kinase C (PKC). Upon stimulation, ERK1/2 are phosphorylated by the upstream kinase MEK on residues Thr202 and Tyr204 and in turn phosphorylate many other downstream molecules that are involved in a range of cellular processes such as cell proliferation, differentiation, motility and cell death.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Synthetic peptide (TGFLT*EY*VATRC) conjugated to KLH.
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- Affinity purified.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 1.0 µg per ml. For immunocytochemistry, a concentration of 1.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2565604 (BioLegend Cat. No. 675502)
Antigen Details
- Structure
- Human ERK1 (MAPK3) and ERK2 (MAPK1) encode 43, 40, 38kD and 41, 36kD protein kinases, respectively. Mouse ERK1 (MAPK3) and ERK2 (MAPK1) encode 43kD and 41kD protein kinases, respectively. ERK1 and ERK2 share high homology.
- Distribution
-
Cytoplasm. Nucleus.
- Function
- ERK1/2 are broadly expressed protein kinases that are involved in proliferation, differentiation, motility, and cell death. In response to extracellular stimuli, ERK1/2 are phosphorylated at Thr202 and Tyr204 and in turn transduce signals to downstream substrates.
- Interaction
- ERK1/2 phosphorylates a wide range of downstream substrates, such as ATF2, BCL6, EIF4EBP1, CANX, MAP2, DAPK1, RAF1, SYK, and GRB10.
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction, Synaptic Biology
- Molecular Family
- Postsynaptic proteins, Phospho-Proteins, Protein Kinases/Phosphatase
- Antigen References
-
1. Futran AS, et al. 2013. Curr. Biol. 23:R972.
2. Mendoza MC, et al. 2011. Trends Biochem. Sci. 36:320.
3. Chambard JC, et al. 2007. Biochim. Biophys. Acta. 1773:1299.
4. Roux PP, et al. 2004. Microbiol. Mol. Biol. Rev. 68:320.
5. Torii S, et al. 2004. J. Biochem. 136:557.
6. Clark EA, et al. 1996. J. Biol. Chem. 271:14814. - Gene ID
- 5594 View all products for this Gene ID
- UniProt
- View information about ERK1/2 on UniProt.org
Other Formats
View All ERK1/2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-ERK1/2 Phospho (Thr202/Tyr204) | 4B11B69 | WB,ICC |
| Direct-Blot™ HRP anti-ERK1/2 Phospho (Thr202/Tyr204) | 4B11B69 | WB |
| Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/Tyr204) | 4B11B69 | ICFC |
| Alexa Fluor® 488 anti-ERK1/2 Phospho (Thr202/Tyr204) | 4B11B69 | ICFC |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-ERK1/2 Phospho (Thr202/Tyr204)
Total cell lysates (15 µg protein) from Jurkat cells without... 
Untreated (left) and PMA-treated (20 nM PMA treatment for 10... -
Direct-Blot™ HRP anti-ERK1/2 Phospho (Thr202/Tyr204)
15 µg of total protein extract from Jurkat cells treated wit... -
Alexa Fluor® 647 anti-ERK1/2 Phospho (Thr202/Tyr204)
Human peripheral blood lymphocytes were stimulated with (fil... 
C57BL/6 mouse splenocytes were stimulated with (filled histo... -
Alexa Fluor® 488 anti-ERK1/2 Phospho (Thr202/Tyr204)
Human peripheral blood lymphocytes were stimulated with (fil...