- Clone
- Poly6242 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Mitogen activated protein kinase 1, extracellular signal regulated kinase 2, MAPK1, p41mapk, p42 MAP Kinase
- Isotype
- Rabbit Polyclonal IgG
-
Whole cell extracts (15 µg protein) from RPMI 8226 (reduced expression control), HeLa, K562, and NIH/3T3 cells were resolved on a 4-12% Bis-Tris gel, transferred to a nitrocellulose membrane and probed with a 1:2000 dilution of Purified anti-ERK2 Antibody, clone Poly6242, overnight at 4°C. Proteins were visualized by chemiluminescence detection HRP donkey anti-rabbit IgG Antibody (Cat. No. 406401) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-Actin Antibody (Cat. No. 664804) was used as a loading control at a 1:25000 dilution (lower). Predicted MAPK1 expression obtained from Human Protein Atlas. Lane M: Molecular Weight marker. -
Formalin-fixed paraffin-embedded human testis tissue was stained with Poly6242 and developed with an alkaline phosphatase chromogen substate (red color). Tissue was counterstained with H&E (blue/pink). Magnification, 40X.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 624201 | 50 µL | $118.00 | |||
ERK2 is a 42 kD dual specificity kinase also known as mitogen activated protein kinase 1, extracellular signal regulated kinase 2, MAPK1, and p41mapk. The MAPK family includes several subgroups encompassing the ERK, JNK, and p38 kinases. ERK is phosphorylated in response to various extracellular signals inducing homodimerization and nuclear translocation where it phosphorylates target proteins regulating cell growth and survival. ERK2 interacts with a number of proteins including STAT5A, estrogen receptor alpha, ELK1, MEK1, protein kinase c epilson, RAF1, ribosomal S6 kinase, VAV1, STAT5b, Shc and many others. The Poly6242 antibody recognizes human, mouse, and rat ERK2 and has been shown to be useful for Western blotting, immunoprecipitation, and immunohistochemistry.
Product Details
- Verified Reactivity
- Rat, Human, Mouse
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Peptide mapping to the carboxy terminus of rat ERK2
- Formulation
- This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide, 50% glycerol, and 0.1% gelatin.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- Lot-specific (to obtain lot-specific concentration, please enter the lot number in our Concentration and Expiration Lookup or Certificate of Analysis online tools.)
- Storage & Handling
- Upon receipt, store frozen at -20°C.
- Application
-
WB - Quality tested
IHC, IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 10 µl per 5 ml of antibody dilution buffer for each mini-gel. For IHC, use a 1:50 dilution of antibody for staining. Antigen retrieval for IHC of formalin-fixed paraffin-embedded tissue using 0.01 M sodium citrate buffer is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This product may contain other non-IgG subtypes. - Additional Product Notes
-
The 50 µL size can be used for approximately 5 Western blots.
- RRID
-
SCR_001134 (BioLegend Cat. No. 624201)
Antigen Details
- Structure
- Mitogen-activated protein kinase family member, dual specificity kinase, homodimer; approximately 42 kD
- Distribution
-
Ubiquitous cytoplasmic protein, translocates to nucleus upon activation.
- Function
- Dual specificity kinase involved in eukaryotic signal transduction. Phosphorylated ERK2 homodimerizes and translocates from the cytoplasm to the nucelus. Activated ERK2 phosphorylates various targets to regulate cell proliferation, survival and apoptosis.
- Interaction
- Interacts with a variety of proteins including STAT5A, estrogen receptor alpha, ELK1, MEK1, protein kinase c epilson, RAF1, ribosomal S6 kinase, VAV1, STAT5b, Shc and many others.
- Modification
- Auto-phosphorylation on multiple sites (threonine, serine, tyrosine)
- Biology Area
- Cell Biology, Signal Transduction
- Molecular Family
- Protein Kinases/Phosphatase
- Antigen References
-
1. Khokhlatchev AV, et al. 1998. Cell 93:605.
2. Boulton TG, et al. 1991. Cell 65:663. - Regulation
- Extracellular signals including cytokine receptor engagement, growth factor engagement, and neuropeptides induce phosphorylation and activation of ERK2.
- Gene ID
- 5594 View all products for this Gene ID
- UniProt
- View information about ERK2 on UniProt.org
Other Formats
View All ERK2 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-ERK2 | Poly6242 | WB,IHC,IP |
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