- Clone
- Poly28601 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FABP1
- Isotype
- Rabbit Polyclonal IgG
-
Western blot of purified anti-FABP1 antibody (Poly28601). Lane 1: Molecular weight marker; Lane 2: 20 µg of human liver lysate; Lane 3: 20 µg of mouse liver lysate; Lane 4: 40 µg of rat liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded human liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded mouse liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm -
IHC staining of purified anti-FABP1 antibody (Poly28601) on formalin-fixed paraffin-embedded rat liver tissue. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 2 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 860101 | 25 µg | $125.00 | |||
Fatty acid binding protein 1 (FABP1) is involved in fatty acid binding, uptake, transport, and metabolism. FABP1 binds to cholesterol, free fatty acids, and bilirubin. Diseases associated with FABP1 include Hepatocellular Adenoma, Fatty Liver Disease and Nonalcoholic 1.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Polyclonal
- Host Species
- Rabbit
- Immunogen
- Recombinant Recombinant Fatty Acid Binding Protein 1 (FABP1) expressed in E.coli.
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 1.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810737 (BioLegend Cat. No. 860101)
Antigen Details
- Structure
- FABP1 is a 127 amino acid protein with an apparent molecular mass of ~ 14 kD.
- Distribution
-
Tissue Distribution: Liver, kidney, colon, rectum
Cellular Distribution: Cytosol and nucleus - Function
- FAPB1 is involved in fatty acid uptake, transport, and metabolism.
- Interaction
- Cholesterol, free fatty acids, bilirubin
- Cell Type
- Mesenchymal cells
- Biology Area
- Cell Biology, Cell Proliferation and Viability
- Molecular Family
- Carrier Proteins
- Antigen References
-
- Amiri M, et al. 2018. Gene. 676:171.
- Xu Y, et al. 2015. Clin Chim Acta. 445:85.
- Thumser AE, et al. 2014. Curr Opin Clin Nutr Metab Care. 17(2):124.
- Gene ID
- 32168 View all products for this Gene ID 14080 View all products for this Gene ID 24360 View all products for this Gene ID
- UniProt
- View information about FABP1 on UniProt.org
Other Formats
View All FABP1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-FABP1 | Poly28601 | WB,IHC-P |
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