- Clone
- 10F7 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- FUS, TLS
- Isotype
- Mouse IgG1, κ
-
Western blot of purified anti-FUS antibody (clone 10F7). Lane 1: Molecular weight marker; Lane 2: 20 μg of human brain lysate; Lane 3: 20 μg of mouse brain lysate; Lane 4: 20 μg of rat brain lysate. The blot was incubated with 5 μg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of Purified anti-FUS (clone 10F7) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., 1X (Cat. No. 928502), the tissue was incubated without (panel A) and with (panel B) 5 µg/mL of Purified anti-FUS (clone 10F7) followed by incubation with Alexa Fluor® 647 Goat anti-mouse IgG (Cat. No. 405322) for 1 hour at room temperature. Nuclei were counterstained with DAPI (Cat. No. 422801). Images were captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 855001 | 25 µg | $106.00 | |||
| 855002 | 100 µg | $276.00 | |||
FUS/TLS is an RNA-binding protein that was originally discovered as a fusion protein caused by chromosomal translocation in cancer. FUS/TLS is as an important member of the cellular DNA repair response. The C-terminal end of FUS is involved in protein and RNA binding, while the N-terminal motif of FUS plays a role in transcriptional activation. FUS was also shown to accumulate in the cytoplasm of neurons under hyperosmotic conditions, and FUS mislocalization has been implicated as being the first step toward disease.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TLS/FUS recombinant protein fused with GST tag.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 1.0 - 5.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2750473 (BioLegend Cat. No. 855001)
AB_2750473 (BioLegend Cat. No. 855002)
Antigen Details
- Structure
- FUS is a 526 amino acid protein with an apparent molecular mass of ~ 53 kD.
- Distribution
-
Tissue Distribution: Ubiquitously expressed
Cellular Distribution: Nucleus and cytosol - Function
- FUS/TLS binds to DNA and maintains genomic integrity.
- Interaction
- Interacts with HDAC1, RNA Polymerase II, FUSIP1, ILF3, PRMT1, RELA, SPI1, and TNPO1.
- Cell Type
- Astrocytes, Neurons, Oligodendrocytes
- Biology Area
- Cell Biology, Neurodegeneration, Neuroscience, Protein Misfolding and Aggregation, Synaptic Biology
- Molecular Family
- Postsynaptic proteins, Presynaptic proteins
- Antigen References
-
- Efimova AD, et al. 2017. Mol Biol (Mosk) 51:387.
- Ederle H, Dormann D. 2017. FEBS Lett. 591:1489.
- Guerrero EN, et al. 2016. Prog. Neurobiol. 145-146:78.
- Gene ID
- 2521 View all products for this Gene ID 233908 View all products for this Gene ID 217385 View all products for this Gene ID
- UniProt
- View information about FUS on UniProt.org
Other Formats
View All FUS Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-FUS | 10F7 | WB,IHC-P |
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