Purified anti-γ-Catenin Antibody

Pricing & Availability
Clone
15F11 (See other available formats)
Regulatory Status
RUO
Other Names
Junction Plakoglobin, DP3, Catenin (Cadherin-associated Protein), Gamma 80kDa, Desmoplakin III, CTNNG, PDGB, PKGB, Catenin Gamma, JUP
Isotype
Mouse IgG1, κ
15F11_PURE_gamma-Catenin_Antibody_1_070921.png
Whole cell extracts (15 µg total protein) from HaCaT and MCF7 (positive controls), and U87-MG and MOLT-4 (negative control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-γ-Catenin antibody (clone 15F11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 15F11_PURE_gamma-Catenin_Antibody_1_070921.png
    Whole cell extracts (15 µg total protein) from HaCaT and MCF7 (positive controls), and U87-MG and MOLT-4 (negative control) cells were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 1.0 μg/mL (1:500 dilution) purified anti-γ-Catenin antibody (clone 15F11) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Western-Ready™ ECL Substrate Premium Kit (Cat. No. 426319) was used as a detection agent. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607904) was used as a loading control at a 1:25000 dilution (lower). Lane M: Molecular weight marker.
  • 15F11_PURE_gamma-Catenin_Antibody_2_070921.png
    IHC staining of purified anti-γ-Catenin antibody (clone 15F11) on formalin-fixed paraffin-embedded human skin (panel A, positive control) and adipose (panel B, negative control) tissues. Following antigen retrieval using Sodium Citrate H.I.E.R (Cat. No. 928502), the tissue was incubated with 5 µg/mL (1:100 dilution) of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin HRP Kit (Multi-Species, DAB, Cat. No. 929501) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. Scale bar: 50 µm.
  • 15F11_PURE_gamma-Catenin_Antibody_3_070921.png
    MOLT-4 cells (negative control) (panel A) and MCF7 cells (positive control) (panel B) were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells were then intracellularly stained with purified anti-γ-Catenin antibody (clone 15F11) overnight at 4°C followed by incubation with Alexa Fluor® 594 goat anti-mouse IgG antibody (Cat. No. 405326) at 2.0 µg/mL. Nuclei were counterstained with DAPI and the image was captured with a 60X objective.
  • 15F11_PURE_gamma-Catenin_Antibody_4_072222
    MCF7 cells (filled histogram, positive control) and U-87 MG cells (open histogram, negative control) were fixed with Fixation Buffer (Cat. No. 420801), permeabilized using True-Phos™ Perm Buffer (Cat. No. 425401), and intracellularly stained with purified anti-γ-catenin antibody (clone 15F11) followed by PE goat anti-mouse IgG antibody (Cat. No. 405307).
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Cat # Size Price Quantity Check Availability
946401 25 µg $118.00
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946402 100 µg $293.00
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Description

γ-catenin, also referred to as JUP and plakoglobin, is a member of the Armadillo family of proteins that are required for Wnt signaling. γ-catenin was originally described as a structural component of desmosomes and adherens junctions, where it links E-cadherins to the actin cytoskeleton. The presence of this complex is critical for cell migration and adhesion. While γ-catenin shows high overall similarity to the homolologous β-catenin, notably in the armadillo domain rich central region, γ-catenin has distinct N- and C-terminal regions, which allows interaction with protein partners functionally distinct with those of other catenins. Additionally, γ-catenin shows tumor suppressor activity by downregulation of oncogenic Wnt signaling. This negative regulation occurs through multiple mechanisms: a. displacement of β-catenin from junctional complexes thereby promoting its ubiquitin-dependent degradation, b. disrupting formation of the TCF- β-catenin transcriptional activator complex by directly binding β-catenin, c. stimulating the nuclear export of the SOX4 transcription factor. γ-catenin also plays a role in maintaining embryonic stem cells in a pluripotent state.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Full-length recombinant chicken γ-catenin
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

WB - Quality tested
IHC-P, ICC, ICFC - Verified
Recommended Usage

Each lot of this antibody is quality control tested by western blotting. For western blotting, the suggested use of this reagent is 0.1 - 1.0 µg/mL. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 5.0 - 25.0 µg/mL is suggested. For immunocytochemistry, a concentration range of 5.0 μg/mL is recommended. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

This clone was tested for ICC on MCF7 (positive control) and MOLT-4 (negative control) cells using multiple fixation and permeabilization conditions. A brief summary of these results is listed below:

  • methanol only: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Moderate nuclear background staining observed in MCF7 cells.
     
  • 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.
     
  • 4% PFA plus methanol: 4% PFA plus Triton X-100: plasma membrane associated staining in MCF7 cells, no staining in MOLT-4 cells. Low nuclear background staining observed in MCF7 cells.
RRID
AB_2894532 (BioLegend Cat. No. 946401)
AB_2894532 (BioLegend Cat. No. 946402)

Antigen Details

Structure
γ-catenin is a 745 amino acid protein with a predicted molecular weight of 82 kD.
Distribution

Ubiquitously expressed/Cytoskeleton and plasma membrane associated

Function
Cell to cell adhesion, cell signaling
Biology Area
Cell Adhesion, Cell Biology, Cell Motility/Cytoskeleton/Structure, Signal Transduction
Antigen References
  1. Zhurinksy J, et al. 2000. J Cell Sci. 18:3127.
  2. Mahendram S, et al. 2013. PLoS ONE. 8:e65320.
  3. Aktary Z, et al. 2017. Oncotarget. 8:32270.
Gene ID
3728 View all products for this Gene ID
UniProt
View information about gamma-Catenin on UniProt.org
Go To Top Version: 2    Revision Date: 07/22/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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