Purified anti-GDI1 Antibody

Pricing & Availability
Clone
Poly28602 (See other available formats)
Regulatory Status
RUO
Other Names
GDP Dissociation Inhibitor 1, Rab GDP dissociation inhibitor alpha, Rab GDI alpha,Guanosine diphosphate dissociation inhibitor 1, GDI-1, Oligophrenin-2, OPHN2, Protein XAP-4, RABGDIA, XAP4
Isotype
Rabbit Polyclonal IgG
Poly28602_Purified_GDI1_Antibody_1_053119
Western blot of purified anti-GDI1 antibody (Poly28602). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain cytosol lysate; Lane 3: 20 µg of mouse brain cytosol lysate; Lane 4: 20 µg of rat brain cytosol lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28602_Purified_GDI1_Antibody_1_053119
    Western blot of purified anti-GDI1 antibody (Poly28602). Lane 1: Molecular weight marker; Lane 2: 20 µg of human brain cytosol lysate; Lane 3: 20 µg of mouse brain cytosol lysate; Lane 4: 20 µg of rat brain cytosol lysate. The blot was incubated with 0.1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled donkey anti-rabbit IgG (Cat. No. 406401). Enhanced chemiluminescence was used as the detection system.
  • Poly28602_Purified_GDI1_Antibody_2_053119
    IHC staining of purified anti-GDI1 antibody (Poly28602) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28602_Purified_GDI1_Antibody_3_053119
    IHC staining of purified anti-GDI1 antibody (Poly28602) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28602_Purified_GDI1_Antibody_4_053119
    IHC staining of purified anti-GDI1 antibody (Poly28602) on formalin-fixed paraffin-embedded mouse brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28602_Purified_GDI1_Antibody_5_053119
    IHC staining of purified anti-GDI1 antibody (Poly28602) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • Poly28602_Purified_GDI1_Antibody_6_053119
    IHC staining of purified anti-GDI1 antibody (Poly28602) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability
860201 25 µg $125.00
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Description

The GDI1 gene encodes Rab GDP dissociation inhibitor alpha (aGDI or GDI), a regulatory protein common to all GTPases of the Rab family. GDI binds to inactive GDP-bound GTPases and sequesters them from the membrane to the cytosol to maintain a soluble pool readily available GTPases. GDI dysfunction leads to abnormal membrane accumulation of various GTPases. Mutations in the GDI1 gene are linked to X-lined mental retardation (XLMR), a common human disorder characterized by mental handicap. Similar to human mental retardation patients, GDI knockout mice have marked defects in synaptic function, particularly related to short-term temporal memory. Mechanistically, lack of GDI impairs synaptic vesicle biogenesis and recycling in the hippocampus.

Technical data sheet

Product Details

Verified Reactivity
Human, Mouse, Rat
Antibody Type
Polyclonal
Host Species
Rabbit
Immunogen
Recombinant GDI1 (Met1-Val274) expressed in E.coli.
Formulation
0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.1 - 2.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 0.5 - 2.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

RRID
AB_2810738 (BioLegend Cat. No. 860201)

Antigen Details

Structure
Human GDI is a 447 amino acid protein with a predicted molecular mass of 50 kD.
Distribution

Tissue distribution: Brain
Cellular distribution: Trans-Golgi network and cytosol

Function
GDI regulates the GDP/GTP exchange reaction in most Rab proteins by inhibiting GDP dissociation from them, and subsequent GTP binding. GDI promotes the dissociation of GDP-bound Rab proteins from the membrane and inhibits their activation.
Interaction
Interacts with RHOH, RAB1A, RAB3A, RAB5A and RAB10.
Cellular targets: GDP-bound Rab proteins
Cell Type
Neurons
Biology Area
Cell Biology, Neuroscience, Synaptic Biology
Molecular Family
Enzymes and Regulators, Golgi Markers
Antigen References
  1. Hutt DM, et al. 2000. J Biol Chem. 275(24):18511-9.
  2. D’Adamo P, et al. 2002. Hum Mol Genet. 11(21):2567-80.
  3. Bianchi V, et al. 2009. Hum Mol Genet. 18(1): 105–117.
Gene ID
2664 View all products for this Gene ID
UniProt
View information about GDI1 on UniProt.org
Go To Top Version: 1    Revision Date: 05/31/2019

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