- Clone
- 12H12 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Gemin3
- Isotype
- Mouse IgG1, κ
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Western blot of purified anti-Gemin 3 antibody (clone 12H12). Lane 1: Molecular weight marker; Lane 2: 20µg of HeLa cell lysate; Lane 3: 10µg of HeLa cell lysate; Lane 4: 5µg of HeLa cell lysate. The blot was incubated with 2 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 865501 | 25 µg | $106.00 | |||
| 865502 | 100 µg | $276.00 | |||
Gemin 3, also known as DEAD-box helicase 20 (DDX20), is a putative RNA helicase that contains a conserved ASP-GLU-ALA-ASP (DEAD) motif. Gemin 3 is involved in a variety of cellular processes including initiation of protein translation, ribosome assembly, and alteration of RNA secondary structure. Gemin 3 is one of the core components of the survival of motor neurons (SMN) macromolecule protein complex, which plays an important role in the assembly and re-structuring of small nucleoproteins and transcriptosomes. Gemin 3 may also be involved in cancer as it acts as a tumor suppressor in hepatocellular carcinoma, and as a tumor promoter in breast cancer and prostate cancer2.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 10 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2810775 (BioLegend Cat. No. 865501)
AB_2810775 (BioLegend Cat. No. 865502)
Antigen Details
- Structure
- Gemin 3 is an 824 amino acid protein with an apparent molecular mass of ~ 92 kD.
- Distribution
-
Tissue Distribution: Testis and ovary
Cellular Distribution: Cytosol, nucleus, and cytoskeleton - Function
- Gemin 3 is a component of SMN macromolecule protein complex.
- Interaction
- SMN, GEMIN4
- Cell Type
- Astrocytes, GABAergic Neurons, Glutamatergic Neurons, Mature Neurons, Microglia, Neural Stem Cells, Neurons, Oligodendrocytes
- Biology Area
- Cancer Biomarkers, Neurodegeneration, Neuroscience, Synaptic Biology, Transcription Factors
- Antigen References
-
- Seng CO, et al. 2015. Hum. Mol. Genet. 24(8):2138.
- Chen W, et al. 2016. Int. J. Mol. Med. 37(6):1551.
- Gene ID
- 11218 View all products for this Gene ID
- UniProt
- View information about Gemin 3 on UniProt.org
Other Formats
View All Gemin 3 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Gemin 3 | 12H12 | WB |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Gemin 3
Western blot of purified anti-Gemin 3 antibody (clone 12H12)...