- Clone
- BL28543 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- GGT1, Gamma-glutamyltransferase 1
- Isotype
- Mouse IgG1, κ
-
Western blot of purified anti-GGT1 antibody (clone BL28543). Lane 1: Molecular weight marker; Lane 2: 20 µg of HepG2 Cell lysate; Lane 3: 20 µg of human liver lysate. The blot was incubated with 0.5 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-GGT1 antibody (clone BL28543) on formalin-fixed paraffin-embedded human kidney tissue. Following antigen retrieval using Sodium Citrate H.I.E.R, the tissue was incubated with 1 µg/ml of the primary antibody overnight at 4°C. BioLegend’s Ultra Streptavidin (USA) HRP Detection Kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale Bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 854301 | 25 µg | $106.00 | |||
GGT1 is a transferase that plays a key role in the gamma-glutamyl cycle. GGT1 is abundantly expressed in liver and kidney. Its involvement in glutathione metabolism makes it an important regulator of the oxidative stress in the central nervous system, as well as in other tissues. GGT1 is also a known marker for apoptosis. Importantly, elevated levels of GGT1 correlates with breast cancer tumor progression and resistance to chemotherapy, suggesting GGT1 as a candidate for cancer prognosis marker.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 1.0 - 5.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2783438 (BioLegend Cat. No. 854301)
Antigen Details
- Structure
- GGT1 is a 569 amino acid protein with a molecular mass of ~ 61 kD.
- Distribution
-
Tissue Distribution: Kidney, liver, pancreas, stomach, intestine, placenta, and lung.
Cellular distribution: Mainly on plasma membrane or extracellular - Function
- Cleaves the gamma-glutamyl bond of extracellular glutathione, maintains intracellular GSH level.
- Interaction
- Heterodimer forms by interactions between light and heavy chains.
- Biology Area
- Cell Biology, Neuroinflammation, Neuroscience, Synaptic Biology
- Molecular Family
- Postsynaptic proteins, Presynaptic proteins
- Antigen References
-
- Priolo C, et al. 2018. Proc Natl Acad Sci U S A. 115(27):E6274
- Darin N, et al. 2018. Eur J Hum Genet. 26(6):808
- Yoshikawa C. 1995. Nihon Rinsho. 53(5): 1162
- Gene ID
- 2678 View all products for this Gene ID
- UniProt
- View information about GGT1 on UniProt.org
Other Formats
View All GGT1 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-GGT1 | BL28543 | WB,IHC-P |
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