Purified anti-GluR1 Antibody (Previously Covance catalog# MMS-5143)

Pricing & Availability
Clone
N355/1 (See other available formats)
Regulatory Status
RUO
Other Names
GluA1/GluR1 glutamate receptor, Glutamate receptor 1, GluR1, gluR-1, gluR-K1, glutamate receptor A, glutamate receptor subunit GluR1, AMPA-selective glutamate receptor 1, glutamate receptor, ionotropic, AMPA1 (alpha 1)
Previously
Covance Catalog# MMS-5143
Isotype
Mouse IgG1, κ
N355slash1_Purified_GluR1_Antibody_1_012615
Western blot of purified anti-GluR1 antibody (clone N355/1). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N355slash1_Purified_GluR1_Antibody_1_012615
    Western blot of purified anti-GluR1 antibody (clone N355/1). Lane 1: Molecular weight marker; Lane 2: 20 µg of mouse brain membrane lysate; Lane 3: 20 µg of rat brain membrane lysate. The blot was incubated with 10 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • N355slash1_Purified_GluR1_Antibody_2_012615
    IHC staining of purified anti-GluR1 antibody (clone N355/1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • N355slash1_Purified_GluR1_Antibody_3_012615
    IHC staining of purified anti-GluR1 antibody (clone N355/1) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective.
Cat # Size Price Quantity Check Availability
819802 25 µL $83.00
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819801 100 µL $165.00
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Description

GluR1 belongs to the family of Glutamate Receptors (GluRs) that are essential for regulating excitatory synaptic transmission by L-glutamate in the CNS of vertebrates. These are mostly expressed in granule and pyramidal cells in the hippocampus. GluRs can be categorized as ionotropic or metabotropic and subcategorized by their specific agonists (NMDA, AMPA or Kainic acid). There are four types of AMPA related GluR subunits (GluR1, GluR2, GluR3 and GluR4) that are generated by alternative RNA splicing. Tetrameric or pentameric combinations of different subunits contribute to the functional diversity of AMPA receptors.

Technical data sheet

Product Details

Verified Reactivity
Mouse, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a fusion protein corresponding to amino acids 1-389 (extracellular, N-terminus) of rat GluA1/GluR1 glutamate receptor.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 5.0 - 10.0 µg per ml. For immunohistochemistry of formalin-fixed paraffin-embedded tissue, a concentration of 10.0 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Brown EA, et al. 2018. Mol Autism. 9:48. PubMed
  2. Lautz JD, et al. 2021. Cell Rep. 37:110076. PubMed
RRID
AB_2564834 (BioLegend Cat. No. 819802)
AB_2564834 (BioLegend Cat. No. 819801)

Antigen Details

Structure
Expected MW: 100 kD
Cell Type
Glutamatergic Neurons
Biology Area
Cell Biology, Neuroscience, Neuroscience Cell Markers, Synaptic Biology
Molecular Family
Postsynaptic proteins
Gene ID
50592 View all products for this Gene ID
UniProt
View information about GluA1/GluR1 glutamate receptor on UniProt.org
Go To Top Version: 5    Revision Date: 07/09/2024

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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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