- Clone
- BL28630 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Zonulin, Alpha Polypeptide, Beta polypeptide, Binding Peptide, HP2ALPHA2, HPA1S, BP, Haptoglobin Alpha(1S)-Beta, Haptoglobin Alpha(2FS)-Beta
- Isotype
- Mouse IgG1, κ
-
Western blot of purified anti-Haptoglobin antibody (clone BL28630). Lane 1: Molecular weight marker; Lane 2: 1 µg of human plasma; Lane 3: 2 µg of human plasma; Lane 4: 5 µg of human plasma; Lane 5: 10 µg of human plasma. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP-labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 863001 | 25 µg | $106.00 | |||
Haptoglobin is a protein that is mainly expressed by hepatic cells in the liver. Haptoglobin is an acute-phase antioxidant that binds to free hemoglobin (Hb) in the plasma and inhibits the oxidative activity of hemoglobin. Individuals carrying the haptoglobin 2-2 genotype have decreased affinity to Hb and are at a higher risk of developing cerebral aneurysm-related complications due to impaired anti-inflammatory pathways. This highlights haptoglobin as part of the Hb scavenging mechanism, which may be critical for clearing Hb after intracranial bleeding and neuroprotection.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant human Haptoglobin corresponding to amino acids 168 – 391 expressed by E.coli.
- Formulation
- 0.01 M PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Store at 4°C for up to 3 months. For longer term storage aliquot into small volumes and store at -20°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1 - 5 µg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2814603 (BioLegend Cat. No. 863001)
Antigen Details
- Structure
- Haptoglobin is a 406 amino acid protein with predicted and observed molecular mass of ~45 kD.
- Distribution
-
Tissue Distribution: Liver and blood plasma
Cellular Distribution: Extracellular and cytosol - Function
- Haptoglobin is a part of the hemoglobin scavenging mechanism that clears Hb released into the central nervous system after intracranial bleeding.
- Interaction
- Haptoglobin forms tetramers of two alpha and two beta chains. It binds to hemoglobin and CD163.
- Biology Area
- Neurodegeneration, Neuroinflammation, Neuroscience
- Molecular Family
- Blood Group Markers
- Antigen References
-
- Bulters D, et al. 2018. Nat Rev Neurol. 14(7):416.
- Blackburn SL, et al. 2018. Front Physiol. 9:592.
- Bamm VV, et al. 2017. Metab Brain Dis. 32(1):19-34.
- Gene ID
- 3240 View all products for this Gene ID
- UniProt
- View information about Haptoglobin on UniProt.org
Other Formats
View All Haptoglobin Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Haptoglobin | BL28630 | WB |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Haptoglobin
Western blot of purified anti-Haptoglobin antibody (clone BL...