- Clone
- A15061E (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Hepatocyte Growth Factor, Scatter Factor (SF), Hepatopoietin (HPTA), Lung fibroblast-derived mitogen
- Isotype
- Mouse IgG2b, κ
-
Total cell lysates (30 µg protein) from U87-MG (lane 1), THP-1 untreated (lane 2), THP-1 treated with PMA for three days and LPS for one day (lane 3), Raw264.7 (lane 4) and 5 ng recombinant human HGF (lane 5) were resolved by 4-12% Bis-Tris gel electrophoresis under non-reducing condition, transferred to nitrocellulose, and probed with purified anti-HGF (clone A15061E) antibody. Proteins were visualized using a goat anti-mouse-IgG secondary antibody conjugated to HRP and chemiluminescence detection. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control. -
Whole cell extracts (15 µg total protein) of indicated cell lines, reduced or non-reduced with βME, were resolved by 4-12% Bis-Tris gel electrophoresis, transferred to a PVDF membrane, and probed with 0.5 mg/mL of purified anti-HGF antibody (clone A15061E) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-β-actin (clone 2F1-1) antibody was used as a loading control at a 1:10000 dilution (lower). Lane M: Molecular weight marker.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 692602 | 100 µg | $253.00 | |||
HGF (Hepatocyte growth factor) is a multifunctional cytokine that plays important roles in cell proliferation, survival, motility and morphogenesis. HGF protects the epithelium, neurons and cardiomyocytes during organ diseases; this protective effect is mediated through anti-apoptotic signals via inhibition of caspase-3 activity or induction of anti-apoptotic molecules. Secreted by cells of mesenchymal origin, HGF is the specific ligand for the tyrosine-kinase receptor c-MET (cellular mesenchymal-epithelial transition), also called MET, which is expressed in different types of epithelial, endothelial and hematopoietic progenitor cells. Aberrantly activated MET/HGF signaling correlates with tumorigenesis and metastasis, and is regarded as a target for novel anti-cancer treatments.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant Human HGF (carrier-free)
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.5 - 2.0 µg per mL. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This antibody works for Western blotting under non-reducing conditions only and does not cross-react with mouse.
- RRID
-
AB_2632766 (BioLegend Cat. No. 692602)
Antigen Details
- Structure
- Heterodimer (alpha and beta chains). Pro-HGF has 728 amino acids, the predicted molecular weight is approximately 83 kD. The alpha and beta chains contain 463 and 234 amino acids and have a predicted molecular mass of approximately 53.6 and 26 kD respec
- Distribution
-
Mesenchymal cells, liver sinusoidal endothelial, and macrophages.
- Function
- Stimulates epithelial cell proliferation, motility, morphogenesis and angiogenesis. Hypoxia and hyperglycemia suppress HGF production. Also, TGF-β and angiotensin-II depress HGF production in vitro.
- Interaction
- Hepathocytes and epithelial cells.
- Ligand/Receptor
- MET receptor.
- Biology Area
- Angiogenesis, Apoptosis/Tumor Suppressors/Cell Death, Cancer Biomarkers, Cell Biology, Cell Proliferation and Viability, Immunology, Signal Transduction
- Molecular Family
- Cytokines/Chemokines, Growth Factors
- Antigen References
-
1. Hartmann S, et al. 2016. Clin. Cancer Res. 22:4005.
2. Fajardo-Puerta AB, et al. 2016. J. Clin. Pathol. 69:575.
3. Zhang Y, et al. 2016. Oncotarget. 7:37370-89.
4. Frisch RN, et al. 2016. Expert Opin. Ther. Targets 20:1087.
5. Kataoka H, et al. 2000. J. Biol. Chem. 275:40453.
6. Wang X, et al. 2002. Mol. Cell. 9:411.
7. Wu MH, et al. 2004. Gene Ther. 11:170.
8. Mizuno S, et al. 2008. Front. Biosci. 13:7072.
9. D'Angelo F, et al. 2013. Clin. Exp. Immunol. 174:60. - Gene ID
- 3082 View all products for this Gene ID
- UniProt
- View information about HGF on UniProt.org
Other Formats
View All HGF Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-HGF | A15061E | WB |
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