- Clone
- 2G1F9 (See other available formats)
- Regulatory Status
- RUO
- Isotype
- Rat IgG2a, κ
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Total cell lysates (15µg protein) from NIH/3T3 and HeLa cells treated with (+) or without (-) 5 mM Na-Butyrate for 20 hours were resolved by 4-20% Tris-Glycine gel electrophoresis, transferred to nitrocellulose, and probed with 1 µg/mL purified anti-K9ac H3 (clone 2G1F9, left) antibody and a competitor’s antibody used at the manufacturer’s recommended concentration (right). Proteins were visualized by chemiluminescence detection using goat anti-rat-IgG (for 2G1F9, cat #405405) or donkey anti-rabbit-IgG secondary antibodies conjugated to HRP (for competitor’s clone, cat #406401). Total Histone H3 (cat# 601902) was used as a loading control (lower). Lane M: Molecular weight ladder. -
Untreated (panel A) and sodium butyrate-treated (5 mM for 16 hours, panel B) HeLa cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.5% Triton X-100 for 10 minutes, and blocked with 5% FBS for 60 minutes. Cells treated were then intracellularly stained with a 1:100 dilution (5.0 µg/mL) of Purified anti-Histone H3 Acetylated Lysine 9 Antibody, clone 2G1F9, overnight at 4°C, after which proteins were visualized with Alexa Fluor® 488 Goat anti-mouse IgG Antibody (Cat. No. 405319) at 1.0 µg/mL. Nuclei were counter-stained with DAPI, and the image was captured with a 60X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 698401 | 25 µg | $101.00 | |||
| 698402 | 100 µg | $253.00 | |||
Histone subunit H3, along with subunits 2A, 2B, and 4, make up the core subunits of the nucleosome octomer. An octomer contains two protomers of each subunit tightly wrapped around a ~147 bp segment of DNA. Histones have integral roles in chromatin integrity, genomic stability, and gene regulation. Post-translational modification of histones in response to certain stimuli results in alterations of nucleosomal positioning relative to DNA.
Product Details
- Verified Reactivity
- Human, Mouse
- Antibody Type
- Monoclonal
- Host Species
- Rat
- Immunogen
- Histone H3 K9ac peptide
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
ICC - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.01 - 1.0 µg per ml (1:500-1:50000 dilution). For immunocytochemistry, a concentration range of 1.0 - 5.0 μg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing).
- RRID
-
AB_2715783 (BioLegend Cat. No. 698401)
AB_2715783 (BioLegend Cat. No. 698402)
Antigen Details
- Structure
- Histone H3 is a 134 aa protein with a predicted molecular weight of 15.4 kD. The subunit is comprised of a histone fold, an αN-helix main globular domain and a large N-terminal tail harboring residues subject to multiple types of post-translational.
- Distribution
-
Nucleus
- Function
- Histone acetylation is generally associated with activation of gene expression, nucleosomal redistribution, and histone deposition. One consequence of this modification is the alteration of chromatin structure to facilitate recruitment of transcription factors. Additionally, acetyl-lysine modification is also used as binding site for proteins containing bromodomains, resulting in their recruitment to the modified histones.
- Interaction
- Histones 2A, 2B, 4, HDAC
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics
- Antigen References
-
1. Zhang, et al. 2017. Nat. Commun. 8: 14799.
2. Kanda M, et al. 2016. Proc. Natl. Acad. Sci. USA 113: E3394.
3. Bellet MM, et al. 2013. Proc. Natl. Acad. Sci. USA 9: 3333.
4. Li B, et al. 2016. Sci. Rep. 6: 26542.
5. Li D, et al. 2014, Mol. Cancer. 13:26. - Gene ID
- 8350 View all products for this Gene ID
- UniProt
- View information about Histone on UniProt.org
Other Formats
View All Histone Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Histone H3 Acetylated Lysine 9 (K9ac) | 2G1F9 | WB,ICC |
| Alexa Fluor® 594 anti-Histone H3 Acetylated Lysine 9 (K9ac) | 2G1F9 | ICC |
| Alexa Fluor® 488 anti-Histone H3 Acetylated Lysine 9 (K9ac) | 2G1F9 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-Histone H3 Acetylated Lysine 9 (K9ac)
Total cell lysates (15µg protein) from NIH/3T3 and HeLa cel... 
Untreated (panel A) and sodium butyrate-treated (5 mM for 16... -
Alexa Fluor® 594 anti-Histone H3 Acetylated Lysine 9 (K9ac)
Untreated (panel A) and sodium butyrate-treated (5 mM for 16... -
Alexa Fluor® 488 anti-Histone H3 Acetylated Lysine 9 (K9ac)
Untreated (panel A) and sodium butyrate-treated (5 mM for 16...