Purified anti-Histone H3 Dimethyl (Lys9) Antibody (Previously Covance catalog# MMS-5078)

Pricing & Availability
Clone
5E5-G5 (See other available formats)
Regulatory Status
RUO
Other Names
Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
Previously
Covance Catalog# MMS-5078
Isotype
Mouse IgG1, κ
5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_1_090518
Western blot of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa nuclear fraction lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_1_090518
    Western blot of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa nuclear fraction lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system.
  • 5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_2_090518
    IHC staining of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_3_090518
    IHC staining of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
  • 5E5-G5_Pure_Histone-H3-Dimethyl_Antibody_4_090518
    IHC staining of purified anti-Histone H3 Dimethyl (Lys9) antibody (clone 5E5-G5) on formalin-fixed paraffin-embedded rat brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
Cat # Size Price Quantity Check Availability
815501 100 µL $335.00
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Description

Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.

Technical data sheet

Product Details

Verified Reactivity
Human, Rat
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, di-methylated at Lysine-9.
Formulation
Phosphate-buffered solution.
Preparation
The antibody was purified by affinity chromatography.
Concentration
1 mg/ml
Storage & Handling
This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
Application

WB - Quality tested
IHC-P - Verified
Recommended Usage

Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 10 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissue, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.

Product Citations
  1. Wimmers F, et al. 2021. Cell. 184:3915. PubMed
RRID
AB_2564793 (BioLegend Cat. No. 815501)

Antigen Details

Structure
Expected MW: 17 kD
Biology Area
Cell Biology, Neuroscience, Signal Transduction
Antigen References
  1. Murzina N, et al. 2008. Structure. 16:1077.
  2. Chen S, et al. 2015. Genes & Dev. 29:1326.
Gene ID
8350 View all products for this Gene ID
UniProt
View information about Histone on UniProt.org
Go To Top Version: 4    Revision Date: 07/09/2024

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