- Clone
- 9B1-2G6 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
- Previously
-
Covance Catalog# MMS-5083
- Isotype
- Mouse IgG1, κ
-
Western blot of purified anti-Histone H3 Nonmethyl (Lys9) antibody (clone 9B1-2G6). Lane 1: Molecular weight marker; Lane 2: 30 µg of HeLa nuclear cell lysate. The blot was incubated with 1 µg/mL of the primary antibody overnight at 4°C, followed by incubation with HRP labeled goat anti-mouse IgG (Cat. No. 405306). Enhanced chemiluminescence was used as the detection system. -
IHC staining of purified anti-Histone H3 Nonmethyl (Lys9) antibody (clone 9B1-2G6) on formalin-fixed paraffin-embedded human brain tissue. Following antigen retrieval using Sodium Citrate H.I.E.R., the tissue was incubated with 10 µg/ml of the primary antibody overnight at 4°C. BioLegend´s Ultra-Streptavidin (USA) HRP kit (Multi-Species, DAB, Cat. No. 929901) was used for detection followed by hematoxylin counterstaining, according to the protocol provided. The image was captured with a 40X objective. Scale bar: 50 µm
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 815701 | 100 µL | $275.00 | |||
Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, non-methylated at Lysine-9.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB - Quality tested
IHC-P - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 1.0 - 5.0 µg per ml. For immunohistochemistry on formalin-fixed paraffin-embedded tissues, a concentration range of 5.0 - 10 µg/ml is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
-
AB_2564795 (BioLegend Cat. No. 815701)
Antigen Details
- Structure
- Expected MW: 17 kD
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction
- Gene ID
- 8350 View all products for this Gene ID
- UniProt
- View information about Histone on UniProt.org
Other Formats
View All Histone Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Histone H3 Nonmethyl (Lys9) | 9B1-2G6 | WB,IHC-P |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Histone H3 Nonmethyl (Lys9)
Western blot of purified anti-Histone H3 Nonmethyl (Lys9) an... 
IHC staining of purified anti-Histone H3 Nonmethyl (Lys9) an...