- Clone
- 11D8 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histone-H3, H3
- Isotype
- Mouse IgG2b, κ
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293T cell extracts were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified anti-H3-pS10 clone 11D8 antibody. Proteins were visualized using an anti-mouse IgG secondary conjugated to HRP and chemiluminescence detection. Lane 1, is inter phase 293T cell extract; lane 2, is M phase 293T cell extract (overnight nocodazole-treated cells). -
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Exponentially growing Hela cells were stained with purified phospor-Histone H3 (Ser10), clone 11D8 antibody, followed by Alexa Fluor® 488 conjugated to anti-mouse IgG and DAPI. The phosphor-Histone staining is specific on metaphase cells only.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 650801 | 25 µg | $176.00 | |||
| 650802 | 100 µg | $411.00 | |||
Histone H3 is phosphorylated at serine 10 during mitosis and is found to be involved in transcriptional activation, chromatin decondensation, and chromosome compaction during cell division, by the action of Aurora kinase and NIMA kinases.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Modified synthetic peptide conjugated to KLH
- Formulation
-
This antibody is provided in phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Previous lots of this product may have been formulated with 0.1% or 0.05% NaN3 instead of 0.09% NaN3. For further information please contact BioLegend Technical Support or Customer Service. - Preparation
- The antibody was purified by antigen-affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- Upon receipt, store undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IF - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by Western blotting. Western blotting, suggested working dilution(s): Use 1 µg per ml antibody dilution buffer for each mini-gel. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The histone H3 pS10 antibody recognizes phosphorylation of human H3 protein at Ser10 residue and has been shown to be useful for Western blotting.
- Product Citations
-
- RRID
-
AB_10896911 (BioLegend Cat. No. 650801)
AB_10896911 (BioLegend Cat. No. 650802)
Antigen Details
- Structure
- H3 is part of the nucleosome, comprised of an octameric complex with H2A, H2B, and H4 proteins.
- Distribution
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Nucleus
- Function
- H3 is a core component of the nucleosome that serves to wrap and compact DNA into chromatin. Histones, therefore, limit the accessibility of DNA, providing mechanisms for transcription regulation, DNA repair and replication, and chromosomal stability.
- Interaction
- Two molecules of H3 form a heterotetramer with two molecules of H4.
- Biology Area
- Cell Biology, Chromatin Remodeling/Epigenetics, DNA Repair/Replication, Transcription Factors
- Molecular Family
- Phospho-Proteins
- Antigen References
-
1. Choi HS, et al. 2005. J. Biol. Chem. 280:13545.
2. Goto H, et al. 2002. Genes Cells 7:11.
3. Garcia BA, et al. 2005. Biochemistry 44:13202.
4. Hans F, et al. 2001. Oncogene 20:3021. - Regulation
- H3 is regulated by acetylation, methylation, citrullination, phosphorylation, and ubiquitination.
- Gene ID
- 8290 View all products for this Gene ID
- UniProt
- View information about Histone on UniProt.org
Other Formats
View All Histone Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| PE anti-Histone H3 Phospho (Ser10) | 11D8 | ICFC |
| Purified anti-Histone H3 Phospho (Ser10) | 11D8 | WB |
| Alexa Fluor® 488 anti-Histone H3 Phospho (Ser10) | 11D8 | ICFC |
| Alexa Fluor® 647 anti-Histone H3 Phospho (Ser10) | 11D8 | ICFC |
| Alexa Fluor® 594 anti-Histone H3 Phospho (Ser10) | 11D8 | ICC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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PE anti-Histone H3 Phospho (Ser10)
HeLa cells were treated with 20 µM of Nocodazole for 24 hour... -
Purified anti-Histone H3 Phospho (Ser10)
293T cell extracts were resolved by electrophoresis, transfe... 
Exponentially growing Hela cells were stained with purified ... 
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Alexa Fluor® 488 anti-Histone H3 Phospho (Ser10)
Hela cells were treated with 20 µM of Nocodazole for 24 hour... -
Alexa Fluor® 647 anti-Histone H3 Phospho (Ser10)
HeLa cells, which were treated with 20 µM of Nocodazole for ... -
Alexa Fluor® 594 anti-Histone H3 Phospho (Ser10)
HeLa cells were fixed with 4% paraformaldehyde (PFA) for 15 ...