- Clone
- 6F12-H4 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Histone H3.1, histone H3/a, histone 1, H3/a, H3 histone family, member A
- Previously
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Covance Catalog# MMS-5079
- Isotype
- Mouse IgG1
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Whole cell extracts (15 µg total protein) from the indicated cell lines were resolved on a 4-12% Bis-Tris gel, transferred to a PVDF membrane, and probed with 1 µg/mL (1:500 dilution) of purified anti-Histone H3 Trimethyl (Lys9) antibody (clone 6F12-H4) overnight at 4°C. Proteins were visualized by chemiluminescence detection using HRP goat anti-mouse IgG antibody (Cat. No. 405306) at a 1:3000 dilution. Direct-Blot™ HRP anti-GAPDH antibody (Cat. No. 607903) was used as a loading control at a 1:25000 dilution. Western-Ready™ ECL Substrate Plus Kit (Cat. No. 426317) was used as a detection agent. Lane M: Molecular weight marker -
Staining of Clone 6F12-H4 on frozen rat brain.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 815601 | 100 µL | $335.00 | |||
Histone H3 is a 17 kD nuclear protein that is a component of an octamer containing pairs of each of four core histones (H2A, H2B, H3, H4). The core histones create nucleosome structure of chromosomal fiber in eukaryotes and are dynamic in gene regulation. The histones exhibit cell cycle-dependent transcriptional regulation except for the variant H3.3 which is cell cycle-independent. Histone H3 can be modified by phosphorylation, acetylation, ubiquitination, ribosylation, and methylation. Histone H3 has been shown to interact with Rsk-2, MSK1, GCN5 family of HATs, HDACs, PARP, SET7/9, and CARM1.
Product Details
- Verified Reactivity
- Human, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- This monoclonal antibody was raised against a synthetic peptide from Human Histone H3, tri-methylated at Lysine-9.
- Formulation
- Phosphate-buffered solution.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 1 mg/ml
- Storage & Handling
- This antibody should be handled aseptically as it is free of preservatives such as Sodium Azide. Store this antibody undiluted between 2°C and 8°C. Please note the storage condition for this antibody has been changed from -20°C to between 2°C and 8°C. You can also check the vial label or CoA to find the proper storage conditions.
- Application
-
WB, IHC-F
- Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting.
The optimal working dilution should be determined for each specific assay condition.
• WB: 1:100 - 1:1000*
• IHC: 1:50 - 1:100
Tissue: Frozen sections
Pretreatment: None
Primary Incubation: 24 hours at 4°C - Application Notes
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This antibody is effective in immunoblotting (WB) and immunohistochemistry (IHC).
* Expected MW: 17 kD
WB Positive Control: HeLa cell lysate
IHC Positive Control: Rat Brain
This clone is not recommended for ChIP (Chromatin Immunoprecipitation) assays (as determined by in-house testing). - Product Citations
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- RRID
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AB_2564794 (BioLegend Cat. No. 815601)
Antigen Details
- Biology Area
- Cell Biology, Neuroscience, Signal Transduction
- Gene ID
- 8350 View all products for this Gene ID
- UniProt
- View information about Histone on UniProt.org
Other Formats
View All Histone Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Histone H3 Trimethyl (Lys9) | 6F12-H4 | WB,IHC-F |
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-Histone H3 Trimethyl (Lys9)
Staining of Clone 6F12-H4 on frozen rat brain. 
Whole cell extracts (15 µg total protein) from the indicated...