- Clone
- 3H3C27 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Heat Shock Protein 90 kD Alpha (Cytosolic), Heat Shock 90 kD Protein 1, Beta, Class A Member 1, HSPC1, HSP86, HSP84, HSP89A, HSPCAL4, LAP2, EL52, HSP90B and HSP90N, HSP90, HSP90a
- Isotype
- Mouse IgG2b, κ
-
Total cell lysate from 293T cells (lane 1), HeLa cells (lane 2), and NIH-3T3 cells (lane 3) were resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal anti-HSP90 (clone 3H3C27) antibody. Proteins were visualized using an anti-mouse-IgG secondary conjugated to HRP and chemiluminescence detection. -
Immunoprecipitation of HSP90 from Hela cell extracts. Lane 1 is 1% input. Immunoprecipitation was performed using protein G resins only (lane 2), mouse IgG isotype control (lane 3), and anti-HSP90 antibody (clone 3H3C27, lane 4). Western blot was performed using anti-HSP90 antibody (clone 3H3C27).
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 675402 | 100 µg | $265.00 | |||
Hsp90 is one of the most abundant molecular chaperones. It modulates the stability and transport of a diverse set of critical factors, which include regulatory, metabolic, organizational, and signaling proteins. There are two major isoforms: α and β. In humans, the Hsp90β isoform is constitutively expressed, whereas the Hsp90α isoform is expressed under stress conditions. The Hsp90 dimer acts in concert with other chaperones and is regulated by a number of co-chaperones/accessory proteins. The upregulation of Hsp90 is associated with tumor progression, the invasion and formation of metastases, and the development of drug resistance. Therefore, Hsp90 has emerged as an important target for cancer treatment.
Product Details
- Verified Reactivity
- Human, Mouse, Rat
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Partial human HSP90β recombinant protein (209-488 a.a.) expressed in E. coli.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
WB - Quality tested
IP - Verified - Recommended Usage
-
Each lot of this antibody is quality control tested by Western blotting. For Western blotting, the suggested use of this reagent is 0.125 - 1.0 µg per ml. For immunoprecipitation, the suggested use of this reagent is 10-30 μg per ml. It is recommended that the reagent be titrated for optimal performance for each application.
- Product Citations
-
- RRID
-
AB_2565512 (BioLegend Cat. No. 675402)
Antigen Details
- Structure
- Hsp90α: 732 amino acids with a predicted molecular weight of approximately 85 kD. Hsp90β: 724 amino acids with a predicted molecular weight of approximately 83 kD.
- Distribution
-
Nucleus and cytoplasm.
- Function
- Hsp90 is one of the most abundant molecular chaperones. It modulates the stability and transport of critical factors that include regulatory, metabolic, organizational, and signaling proteins.
- Interaction
- NR3C1, FKBP4, FKBP5, PPID, PPP5C, STIP1, AHSA1, FNIP1, HSF1, SMYD3, TOM34, TERT, PTGES3, CHORDC1, DNAJC7, STUB1, UBE2N, PPP5C, PPP5C, p53/TP53, and CDK6.
- Biology Area
- Cell Biology, Cell Cycle/DNA Replication, DNA Repair/Replication, Neurodegeneration, Neuroinflammation, Neuroscience, Protein Trafficking and Clearance, Signal Transduction
- Molecular Family
- Heat Shock Proteins
- Antigen References
-
1. Barrott JJ, et al. 2013. FEBS J. 280:1381.
2. Gorska M, et al. 2012. Front. Biosci. (Landmark Ed) 17:2269.
3. Barginear MF, et al. 2008. Curr. Cancer Drug Targets 8:522.
4. Arya R, et al. 2007. J. Biosci. 32:595.
5. Neckers L, et al. 2003. Curr. Opin. Oncol. 15:419.
6. Nemoto T, et al. 1997. J. Biol. Chem. 272:26179. - Gene ID
- 3320 View all products for this Gene ID 3326 View all products for this Gene ID
- UniProt
- View information about Hsp90alpha/beta on UniProt.org
Other Formats
View All Hsp90α/β Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-Hsp90α/β | 3H3C27 | WB,IP |
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