- Clone
- 16G5 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- OSF-4, Osteoblast cadherin, OB-cadherin, CDH11, CDHOB, CAD11
- Isotype
- Mouse IgG1, κ
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Human lung cancer cell line H460 cells were stained with purified Cadherin 11 (clone 16G5) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by anti-mouse IgG PE. -
Immunofluorescence analysis. H460 cells were fixed with 4% paraformaldehyde (PFA) and blocked with 5% FBS. Then the cells were stained with 5 µg/ml anti-Cadherin 11 antibody followed by 2 µg/mL Alexa Fluor® 594 conjugated anti-mouse IgG. Nuclei were counterstained with DAPI and are shown in blue. The image was captured with a 60X objective. -
Protein (15 µg) harvested from HeLa, PC3, H460, and NIH3T3 was resolved by electrophoresis, transferred to nitrocellulose, and probed with purified monoclonal Cadherin 11 (clone 16G5) antibody. Proteins were visualized using an HRP Goat anti-mouse IgG (minimal x-reactivity) antibody and chemiluminescence detection. -
Human paraffin-embedded ovary tissue slice was stained with purified anti-human Cadherin 11antibody overnight followed by Alexa Fluor® 594 goat anti-mouse secondary antibody (red). The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 368702 | 100 µg | $265.00 | |||
Cadherins are calcium-dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells. Cadherin 11 is a type I transmembrane glycoprotein. It is a type II (atypical) cadherin, based on its lack of a HAV cell adhesion recognition sequence that is specific to type I cadherins. The expression of this particular cadherin in osteoblastic cell lines along with its upregulation during differentiation suggests a specific function in bone development and maintenance. This cadherin is also expressed in some cancer cells.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Recombinant protein containing the extracellular cadherin domain 1 of Cadherin 11 fused to maltose binding protein.
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
ICC, WB, IHC-P - Verified
IP - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 1.0 µg per million cells in 100 µl volume. For Western blotting, the suggested use of this reagent is 1.0 - 2.0 µg/ml. For immunocytochemistry, a concentration rage of 2.0 - 5.0 µg/ml is recommended. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications (for the relevant formats) include: immunoprecipitation.
- Product Citations
-
- RRID
-
AB_2566264 (BioLegend Cat. No. 368702)
Antigen Details
- Structure
- Type I single pass transmembrane glycoprotein.
- Distribution
-
Osteoblasts, neuroblasts, and some cancer cells.
- Function
- Cell adhesion.
- Interaction
- Cadherin 2 (CDH2).
- Ligand/Receptor
- Calcium.
- Cell Type
- Osteoclasts
- Biology Area
- Cell Adhesion, Cell Biology, Immunology, Neuroscience, Synaptic Biology
- Molecular Family
- Adhesion Molecules
- Antigen References
-
1. Wheelock MJ, Johnson KR. 2003. Annu. Rev. Cell Dev. Biol. 19:207-35.
2. Christofori G, et al. 2003. EMBO. J. 22:2318.
3. Okazaki M, et al. 1994. J. Biol. Chem. 269:12092. - Gene ID
- 1009 View all products for this Gene ID
- UniProt
- View information about Cadherin 11 on UniProt.org
Other Formats
View All Cadherin 11 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human Cadherin 11 | 16G5 | FC,ICC,WB,IHC-P,IP |
| APC anti-human Cadherin 11 | 16G5 | FC |
| PE anti-human Cadherin 11 | 16G5 | FC |
| PE/Cyanine7 anti-human Cadherin 11 | 16G5 | FC |
| Alexa Fluor® 647 anti-human Cadherin 11 | 16G5 | IHC-P |
| TotalSeq™-C0131 anti-human Cadherin 11 | 16G5 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human Cadherin 11
Human lung cancer cell line H460 cells were stained with pur... 
Immunofluorescence analysis. H460 cells were fixed with 4% p... 
Protein (15 µg) harvested from HeLa, PC3, H460, and NIH3T3 w... 
Human paraffin-embedded ovary tissue slice was stained with ... -
APC anti-human Cadherin 11
Human lung cancer cell line H460 cells were stained with Cad... -
PE anti-human Cadherin 11
Human lung cancer cell line H460 cells were stained with Cad... -
PE/Cyanine7 anti-human Cadherin 11
Human lung cancer cell line H460 cells were stained with Cad... -
Alexa Fluor® 647 anti-human Cadherin 11
Human paraffin-embedded ovary tissue slices were prepared wi... -
TotalSeq™-C0131 anti-human Cadherin 11