Purified anti-human CD117 (c-kit) Antibody

Pricing & Availability
Clone
S18022G (See other available formats)
Regulatory Status
RUO
Other Names
Stem cell factor receptor, c-kit, mast cell growth factor receptor, steel factor receptor
Isotype
Mouse IgG2a, κ
S18022G_PURE_CD117_c-kit_Antibody_020521
Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with purified anti-human CD117 (c-kit) (clone S18022G) (closed histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
  • S18022G_PURE_CD117_c-kit_Antibody_020521
    Kasumi-3 cells (an acute myeloblastic leukemia cell line) were stained with purified anti-human CD117 (c-kit) (clone S18022G) (closed histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by goat anti-mouse IgG PE.
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Cat # Size Price Quantity Check Availability
375202 100 µg $176.00
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Description

CD117 is a 145 kD protein tyrosine kinase also known as c-kit. It is a receptor for stem cell factor or c-kit ligand. CD117 is expressed on pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, and acute myeloid leukemia cells (AML). CD117 binding of c-kit ligand induces phosphorylation of CD117 and stimulates proliferation and survival of primitive hematopoietic stem cells as well as erythroid-committed and granulo-monocytic committed cells.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD117 transfectant
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S18022G antibody can completely block the binding of clone 104D2 to the target, but it does not block the binding of clone w18195C to the target. Clone S18022G antibody binding to the target is not affected after cells are fixed by 4% PFA.

RRID
AB_2890807 (BioLegend Cat. No. 375202)

Antigen Details

Structure
Growth factor receptor with tyrosine kinase activity, subclass III, approximately 145 kD
Distribution

Pluripotent hematopoietic progenitor cells (approximately 1-4% bone marrow cells), mast cells, acute myeloid leukemic cells (AML)

Function
Receptor for stem cell factor. Induces proliferation and survival of primitive hematopoietic progenitors. Potent inducer of proliferation in erythroid-committed progenitor cells. Defects in CD117 have been linked to severe anemia and a decreased number of hematopoietic progenitor cells.
Ligand/Receptor
Stem cell factor (c-kit ligand )
Antigen References

1. Giebel LB, et al. 1992. Oncogene 7:2207.
2. Furitsu T, et al. 1993. J. Clin. Invest. 92:1736.

Gene ID
3815 View all products for this Gene ID
UniProt
View information about CD117 on UniProt.org
Go To Top Version: 2    Revision Date: 07/08/2021

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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