Purified anti-human CD172a (SIRPα) Antibody

Pricing & Availability
Clone
15-414 (See other available formats)
Regulatory Status
RUO
Other Names
BIT, MFR, P84, MYD-1, SHPS1, PTPNS1, CD172 antigen-like family member A
Isotype
Mouse IgG2a, κ
15-414_PURE_CD172a_Antibody_FC_092016
Human peripheral blood monocytes were stained with purified CD172a (clone 15-414, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
  • 15-414_PURE_CD172a_Antibody_FC_092016
    Human peripheral blood monocytes were stained with purified CD172a (clone 15-414, filled histogram) or purified mouse IgG2a, κ isotype control (open histogram), followed by anti-mouse IgG PE.
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372102 100 µg $288.00
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Description

CD172a, also known as signal-regulatory protein α (SIRPα), is a 85-90 kD, type I transmembrane protein with one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, and two ITIM motifs and a proline-rich region in the cytoplasmic tail. SIRPα is involved in the negative regulation of receptor tyrosine kinase-coupled signaling; the phospho-tyrosine residues of this protein recruit SH2 domain-containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. CD172a is primarily expressed on monocytes/macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells. The ligands of CD172a are CD47, SP-A, and SP-D.

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
KG1a cells.
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤1.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application References

(PubMed link indicates BioLegend citation)
  1. Majdic O, et al. 1981. Blood. 58:1127.
Product Citations
  1. Roussel M, et al. 2021. Cell Reports Medicine. 2(6):100291. PubMed
RRID
AB_2629807 (BioLegend Cat. No. 372102)

Antigen Details

Structure
Type I transmembrane protein, 85-90 kD, one V-set Ig-like and two C-set Ig-like domains in the extracellular portion, two ITIM motifs and a proline-rich region in the cytoplasmic tail.
Distribution

Monocytes, macrophages, granulocytes, dendritic cells, neurons and by cardiomyocytes derived from human pluripotent stem cells.

Function
Negative regulation of receptor tyrosine kinase-coupled signaling, mediates macrophage multinucleation, involved in neuronal survival.
Ligand/Receptor
CD47, SP-A, and SP-D.
Cell Type
Dendritic cells, Granulocytes, Macrophages, Monocytes, Neurons
Biology Area
Cell Biology, Immunology, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Lv Z, et al. 2015. J. Immunol. 195:661.
2. Hatherley D, et al. 2014. J. Biol. Chem. 289:10024.
3. Zen K, et al. 2013. Nat. Commun. 4:2436.
4. Baba N, et al. 2013. J. Exp. Med. 210:1251.
5. Barclay AN, Brown MH. 2006. Nat. Rev. Immunol. 6:457.

Gene ID
140885 View all products for this Gene ID
UniProt
View information about CD172alpha on UniProt.org
Go To Top Version: 1    Revision Date: 09/22/2016

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This data display is provided for general comparisons between formats.
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