- Clone
- H44 (See other available formats)
- Regulatory Status
- RUO
- Workshop
- HCDM listed
- Other Names
- IL-18 receptor alpha chain, IL-18 receptor 1, IL-18 receptor related protein, CDw218a
- Isotype
- Mouse IgG1, κ
-
Human peripheral blood lymphocytes stained with purified H44, followed by biotinylated anti-mouseIgG and Sav-PE
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 313804 | 100 µg | $159.00 | |||
IL-18 receptor is composed of an α and a β subunit that combine to form a high affinity receptor for IL-18. IL-18 receptor α chain, also known as CDw218a, is a 75-80 kD type I transmembrane protein. It is expressed on NK cells, neutrophils, endothelial cells, and subsets of T and B cells. The expression of CDw218a on lymphocytes is upregulated after activation. The interaction of IL-18 and IL-18 receptor has been reported to be implicated in promotion of Th1 cytokine production and atherogenesis.
Product Details
- Verified Reactivity
- Human, Cynomolgus, Rhesus
- Reported Reactivity
- African Green, Baboon
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human NK cell line NK0 constitutively expressing IL-18 receptors
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/ml
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
IHC-F - Reported in the literature, not verified in house - Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤2.0 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
The H44 antibody is specific for IL-18 receptor a chain. Additional reported applications (for the relevant formats) include: immunohistochemistry of acetone-fixed frozen sections and neutralization1.
-
Application References
(PubMed link indicates BioLegend citation) -
- Kitasato Y, et al. 2004. Am. J. Respir. Cell Mol. Biol. 31:619. (IHC)
- Vermot-Desroches C, et al. 2005. Cell Immunol. 236:101. (FC)
- Product Citations
-
- RRID
-
AB_345312 (BioLegend Cat. No. 313804)
Antigen Details
- Structure
- Type I membrane protein, IL-1 receptor family, approximately 75-80 kD
- Distribution
-
Expressed on NK cells and neutrophils, IL-12 activated T and B cells, endothelial cells, and smooth muscle cells; highly expressed on Hodgkin's disease cell lines
- Function
- Binds IL-18 with low affinity; high affinity binding site formed with IL-18Rβ
- Ligand/Receptor
- IL-18
- Cell Type
- B cells, Endothelial cells, Neutrophils, NK cells, T cells
- Biology Area
- Immunology, Innate Immunity
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Torigoe K, et al. 1997. J. Biol. Chem. 272:25737.
2. Gerdes N, et al. 2002. J. Exp. Med. 195:245.
3. Airoldi I, et al. 2000. J. Immunol. 165:6880. - Gene ID
- 8809 View all products for this Gene ID
- UniProt
- View information about CD218a on UniProt.org
Other Formats
View All CD218a Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD218a (IL-18Rα) | H44 | FC,IHC-F |
| Biotin anti-human CD218a (IL-18Rα) | H44 | FC |
| PE anti-human CD218a (IL-18Rα) | H44 | FC |
| FITC anti-human CD218a (IL-18Rα) | H44 | FC |
| PE/Cyanine7 anti-human CD218a (IL-18Rα) | H44 | FC |
| APC anti-human CD218a (IL-18Rα) | H44 | FC |
| TotalSeq™-A0862 anti-human CD218a (IL-18Rα) | H44 | PG |
| TotalSeq™-C0862 anti-human CD218a (IL-18Rα) | H44 | PG |
| TotalSeq™-B0862 anti-human CD218a (IL-18Rα) | H44 | PG |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD218a (IL-18Rα)
Human peripheral blood lymphocytes stained with purified H44... -
Biotin anti-human CD218a (IL-18Rα)
Human peripheral blood lymphocytes stained with biotinylated... -
PE anti-human CD218a (IL-18Rα)
Human peripheral blood lymphocytes stained with H44 PE -
FITC anti-human CD218a (IL-18Rα)
Human peripheral blood lymphocytes stained with H44 FITC -
PE/Cyanine7 anti-human CD218a (IL-18Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
APC anti-human CD218a (IL-18Rα)
Human peripheral blood lymphocytes were stained with CD3 FIT... -
TotalSeq™-A0862 anti-human CD218a (IL-18Rα)
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TotalSeq™-C0862 anti-human CD218a (IL-18Rα)
-
TotalSeq™-B0862 anti-human CD218a (IL-18Rα)