- Clone
- QA20B11 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Glycophorin A/B, GPA/GPB
- Isotype
- Mouse IgG1, κ
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Human red blood cells were stained with purified anti-human CD235ab recombinant (clone QA20B11) (filled histogram) or mouse IgG1, κ isotype control (open histogram) followed by FITC anti-mouse IgG. -
Human paraffin-embedded placenta tissue slices were prepared with a standard protocol of deparaffination and rehydration. Antigen retrieval was done with citrate buffer 1X (10 mM, pH6.0) at 95°C for 40 minutes. The tissue was washed with PBS/ 0.05% Tween-20 twice for five minutes and blocked with 5% FBS and 0.2% gelatin for 30 minutes. Then, the tissue was stained with 5 µg/mL purified anti-human CD235ab recombinant (clone QA20B11) at 4°C overnight. On the next day, the tissue was washed twice with PBS and stained with Alexa Fluor® goat anti-mouse IgG (clone Poly4053) (red) for two hours at room temperature. The nuclei were counterstained with DAPI (blue). The image was captured with a 10X objective.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 382002 | 100 µg | $94.00 | |||
The clone QA20B11 antibody reacts with a common epitope of glycophorin A (CD235a) and glycophorin B (CD235b). Glycophorin A is a major sialoglycoprotein expressed on red blood cell membrane, and erythroid precursors. Glycophorin A shares strong homology with glycophorin B. The clone QA20B11 antibody recognizes human RBCs and erythroid precursors and is useful in erythroid cell development studies. Mature, non-nucleated red blood cells are characteristically glycophorin A positive, but CD45 and CD71 negative.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
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FC - Quality tested
IHC-P - Verified - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.001 µg per million cells in 100 µL volume. For immunohistochemistry on formalin-fixed paraffin-embedded tissue sections, a concentration range of 2.5 - 10.0 µg/mL is suggested. It is recommended that the reagent be titrated for optimal performance for each application.
- RRID
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AB_2924579 (BioLegend Cat. No. 382002)
Antigen Details
- Structure
- Sialoglycoprotein, 20 kD
- Distribution
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Erythrocytes
- Cell Type
- Erythrocytes
- Biology Area
- Immunology
- Molecular Family
- CD Molecules
- Gene ID
- 2993 View all products for this Gene ID
- UniProt
- View information about CD235ab on UniProt.org
Other Formats
View All CD235ab Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD235ab Recombinant Antibody | QA20B11 | FC,IHC-P |
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Purified anti-human CD235ab Recombinant Antibody
Human red blood cells were stained with purified anti-human ... 
Human paraffin-embedded placenta tissue slices were prepared...