Purified anti-human CD25 Antibody

Pricing & Availability
Clone
S20019C (See other available formats)
Regulatory Status
RUO
Other Names
interleukin-2 receptor alpha chain, interluekin-2 receptor, IL2RA, IL-2Rα
Isotype
Mouse IgG1, κ
S20019C_PURE_CD25_Antibody_122722
PHA-activated human peripheral blood lymphocytes were stained with purified anti-human CD25 (clone S20019C) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed anti-mouse IgG PE.
  • S20019C_PURE_CD25_Antibody_122722
    PHA-activated human peripheral blood lymphocytes were stained with purified anti-human CD25 (clone S20019C) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram) followed anti-mouse IgG PE.
  • S20019C_PURE_CD25_Antibody_2_122722
    Human peripheral blood lymphocytes were stained with purified anti-human CD25 (clone S20019C) (left) or purified mouse IgG1, κ (right) followed by goat anti-mouse IgG PE. Cells were costained with anti-human CD4 APC.
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Cat # Size Price Quantity Check Availability
385602 100 µg $107.00
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Description

CD25 is a 55 kD transmembrane protein also known as interleukin-2 receptor alpha chain. CD25 in combination with CD122 and CD132 form a high affinity IL-2 receptor that binds to IL-2, playing a crucial role in activation of JAK1/JAK3 and STAT5 signaling. It is expressed on activated T-Cells as well as on CD4+ T regulatory cells, some progenitor B-cells and activated monocytes. 

Technical data sheet

Product Details

Verified Reactivity
Human
Antibody Type
Monoclonal
Host Species
Mouse
Immunogen
Human CD25 transfected cells
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/mL
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Clone S20019C can completely block binding of clone BC96 and clone TÜ69 on target cells and can be blocked by BC96 and by TÜ69.

Clone S20019C does not block the binding of clone S20019D and clone M-A251 on target cells.

Clone S20019C can block IL-2 binding to receptor.

Clone S20019C shows comparable CD25 staining on 4% formaldehyde-fixed cells compared to unfixed.

RRID
AB_2936548 (BioLegend Cat. No. 385602)

Antigen Details

Structure
55 kD transmembrane protein
Distribution

Activated T-Cells, T regulatory Cells, monocytes

Function
IL-2 receptor
Interaction
CD122, CD132
Ligand/Receptor
IL-2
Cell Type
Lymphocytes, T cells, Tregs
Biology Area
Cell Biology, Immunology
Molecular Family
CD Molecules, Cytokine/Chemokine Receptors
Antigen References
  1. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  2. Waldmann T. 1991. J Biol. Chem. 266:2681-4.
  3. Sakaguchi S, et al. 1995. The Journal of Immunology. 155:1151-64.
Gene ID
3559 View all products for this Gene ID
UniProt
View information about CD25 on UniProt.org
Go To Top Version: 1    Revision Date: 12/27/2022

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This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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