- Clone
- QA18A45 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- IL-2Rα, Low affinity IL-2R, IL-2R α chain, Tac, p55
- Isotype
- Mouse IgG1, κ
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PHA stimulated (3days) human peripheral blood lymphocytes were stained with purified anti-human CD25 recombinant (clone QA18A45) (left) or purified mouse IgG1a, κ isotype control (right), followed by goat anti-mouse IgG PE. Cells were costained with CD3 APC.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 311702 | 100 µg | $112.00 | |||
CD25 is a 55 kD type I transmembrane glycoprotein also known as IL-2 receptor α chain or Tac. It is expressed on progenitor lymphocytes, activated T and B cells, and activated monocytes/macrophages. CD25 is also expressed on a subset of non-stimulated CD4+ T cells termed T regulatory cells. CD25 associates with the IL-2 receptor β (CD122) and common γ chains (CD132) to form the high affinity IL-2R complex.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
QA18A45 clone of antibody is able to completely block the binding of clone BC96 to IL-2Rα.
- RRID
-
AB_2888839 (BioLegend Cat. No. 311702)
Antigen Details
- Structure
- Type I transmembrane glycoprotein, 55 kD
- Distribution
-
Activated T cells and B cells, monocytes/macrophages, Treg
- Function
- Binds IL-2
- Interaction
- IL-2 receptor β (CD122) and γ chains (CD132) to form high affinity IL-2R complex
- Ligand/Receptor
- IL-2
- Cell Type
- Lymphocytes, T cells, Tregs
- Biology Area
- Adaptive Immunity, Cell Proliferation and Viability, Immuno-Oncology, Immunology
- Molecular Family
- CD Molecules, Cytokine/Chemokine Receptors
- Antigen References
-
1. Taniguchi T, et al. 1993. Cell 73:5.
2. Waldmann T. 1991. J. Biol. Chem. 266:2681. - Gene ID
- 3559 View all products for this Gene ID
- UniProt
- View information about CD25 on UniProt.org
Other Formats
View All Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD25 Recombinant Antibody | QA18A45 | FC |
Compare Data Across All Formats
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Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
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Purified anti-human CD25 Recombinant Antibody
PHA stimulated (3days) human peripheral blood lymphocytes we...