- Clone
- 11H3 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- TACI, TNFRSF13B
- Isotype
- Mouse IgG2a, κ
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Human peripheral blood lymphocytes were stained with CD19 APC and purified CD267 (clone 11H3) (left) or purified mouse IgG2a, κ isotype control (right), followed by anti-mouse IgG PE.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 398602 | 100 µg | $206.00 | |||
TACI, Transmembrane Activator CAML (calcium modulator and cyclophilin ligand) Interactor, is a 32 kD type III transmembrane protein. It belongs to TNF receptor superfamily, known as TNFRSF member 13B (TNFRSF13B) or CD267. TACI is expressed on B cells, predominantly CD27+ memory B cells, and myeloma cells. TACI contains 2 cysteine-rich domains (CRDs) and 1 potential N-linked glycosylation site in the extracellular domain. Recent studies, however, have shown that another shorter form (TACI_d2) of TACI exists wherein the N-terminal CRD is removed by alternative splicing. TACI_d2 contains full affinity for its ligands. Several proteins (BAFF/BLys, APRIL, Syndecan-2) have been identified as TACI ligands. The interaction of TACI with its ligands induces activation of the transcription factors NFAT, AP1, and NF-κ B. TACI also plays a crucial role in humoral immunity by negative regulation of B cell proliferation and survival.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Monoclonal
- Host Species
- Mouse
- Immunogen
- Human TACI transfected cells
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
Activation, WB - Reported in the literature, not verified in house - Recommended Usage
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Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Additional reported applications include: western blot5 and inducing of isotype switch signaling in B cells1, suppressing Ab production through TACI signaling2, inducing phosphorylation of PKA in B cells3.
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Application References
(PubMed link indicates BioLegend citation) -
- Martinez-Gallo M, et al. 2013. J Allergy Clin Immunol. 131: 468-76. (Activation)
- Sakurai D, et al. 2007. Eur. J Immunol. 37: 110-8. (Activation)
- Sakurai D, et al. 2007. Blood. 109: 2961-7. (Activation)
- He B, et al. 2010. Nat. Immunol. 11: 836-45. (FC)
- Garcia-Carmona Y, et al. 2018. Front Immunol. 9: 2125. (WB)
- RRID
-
AB_2820066 (BioLegend Cat. No. 398602)
Antigen Details
- Structure
- A 32 kD type III transmembrane protein and belongs to TNF receptor superfamily, known as TNFRSF member 13B (TNFRSF13B) or CD267. TACI contains 2 cysteine-rich domains (CRDs) and 1 potential N-linked glycosylation site in the extracellular domain. Recent studies, however, have shown that another shorter form (TACI_d2) of TACI exists wherein the N-terminal CRD is removed by alternative splicing.
- Distribution
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Expressed on B cells, predominantly CD27+ memory B cells, and myeloma cells.
- Function
- Induces activation of the transcription factors NFAT, AP1, and NF-kB and plays a crucial role in humoral immunity by negative regulation of B cell proliferation and survival.
- Cell Type
- B cells
- Biology Area
- Immunology, Signal Transduction
- Molecular Family
- CD Molecules, Immune Checkpoint Receptors
- Antigen References
-
- Gross JA, et al. 2000. Nature 404:995.
- Wu Y, et al. 2000. J. Biol Chem. 275:35478.
- Yan M, et al. 2001. Nat. Immunol. 2:638.
- Hymowitz A, et al. 2005. J. Biol. Chem. 280:7218.
- Bischof D, et al. 2006. BLOOD. 107:3235.
- Gene ID
- 23495 View all products for this Gene ID
- UniProt
- View information about CD267 on UniProt.org
Other Formats
View All CD267 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD267 (TACI) | 11H3 | FC |
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Purified anti-human CD267 (TACI)
Human peripheral blood lymphocytes were stained with CD19 AP...