- Clone
- QA20A75 (See other available formats)
- Regulatory Status
- RUO
- Other Names
- Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, TROP1
- Isotype
- Mouse IgG1, κ
-
Human colon carcinoma cell line HT29 were stained with purified anti-human CD326 (EpCAM) recombinant (clone QA20A75) (filled histogram) or purified mouse IgG1, κ isotype control (open histogram), followed by FITC goat-anti-mouse IgG.
| Cat # | Size | Price | Quantity Check Availability | ||
|---|---|---|---|---|---|
| 377502 | 100 µg | $206.00 | |||
CD326 is also known as Ep-CAM, tumor associated calcium signal transducer 1, epithelial cell surface antigen, epithelial glycoprotein 2, EGP2, adenocarcinoma associated antigen, and TROP1. CD326 is a type I transmembrane protein containing six disulfide bridges and one THYRO domain. This cell surface glycosylated 40 kD protein is highly expressed in bone marrow, colon, lung, and most normal epithelial cells and is expressed on carcinomas of gastrointestinal origin. It has been reported that CD326 expression occurs during the early steps of erythrogenesis. CD326 functions as a homotypic calcium-independent cell adhesion molecule and is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation. CD326 antigen is an immunotherapeutic target for the treatment of human carcinomas.
Product Details
- Verified Reactivity
- Human
- Antibody Type
- Recombinant
- Host Species
- Mouse
- Formulation
- Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide
- Preparation
- The antibody was purified by affinity chromatography.
- Concentration
- 0.5 mg/mL
- Storage & Handling
- The antibody solution should be stored undiluted between 2°C and 8°C.
- Application
-
FC - Quality tested
- Recommended Usage
-
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.5 µg per million cells in 100 µL volume. It is recommended that the reagent be titrated for optimal performance for each application.
- Application Notes
-
Clone QA20A75 can completely block the binding of clone 9C4 to target cells.
- RRID
-
AB_2922602 (BioLegend Cat. No. 377502)
Antigen Details
- Structure
- Type I transmembrane protein, contains six disulfide bridges, one THYRO domain, approximate molecular weight 40 kD.
- Distribution
-
Highly expressed in bone marrow, colon, lung, and most normal epithelial cells. Also highly expressed on carcinomas of gastrointestinal origin. Expressed during early erythrogenesis.
- Function
- Homotypic calcium-independent cell adhesion. CD326 is believed to be involved in carcinogenesis by its ability to induce genes involved in cellular metabolism and proliferation.
- Interaction
- Human
- Cell Type
- Embryonic Stem Cells, Epithelial cells
- Biology Area
- Cell Biology, Immunology, Stem Cells
- Molecular Family
- Adhesion Molecules, CD Molecules
- Antigen References
-
- Strnad J, et al. 1989. Cancer Res. 49:314-7.
- Munz M, et al. 2004. Oncogene. 23:5748-58.
- Rao CG, et al. 2005. Int J Oncol. 27:49-57.
- Gene ID
- 4072 View all products for this Gene ID
- UniProt
- View information about CD326 on UniProt.org
Other Formats
View All CD326 Reagents Request Custom Conjugation| Description | Clone | Applications |
|---|---|---|
| Purified anti-human CD326 (EpCAM) Recombinant Antibody | QA20A75 | FC |
| APC anti-human CD326 (EpCAM) Recombinant Antibody | QA20A75 | FC |
| FITC anti-human CD326 (EpCAM) Recombinant Antibody | QA20A75 | FC |
| PE anti-human CD326 (EpCAM) Recombinant Antibody | QA20A75 | FC |
Compare Data Across All Formats
This data display is provided for general comparisons between formats.
Your actual data may vary due to variations in samples, target cells, instruments and their settings, staining conditions, and other factors.
If you need assistance with selecting the best format contact our expert technical support team.
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Purified anti-human CD326 (EpCAM) Recombinant Antibody
Human colon carcinoma cell line HT29 were stained with purif... -
APC anti-human CD326 (EpCAM) Recombinant Antibody
Human colon carcinoma cell line HT29 were stained with anti-... -
FITC anti-human CD326 (EpCAM) Recombinant Antibody
Human colon carcinoma cell line HT29 were stained with anti-... -
PE anti-human CD326 (EpCAM) Recombinant Antibody
Human colon carcinoma cell line HT29 were stained with anti-...