Purified anti-human CD40 Antibody

Pricing & Availability
Clone
HB14 (See other available formats)
Regulatory Status
RUO
Workshop
V CD40.5
Other Names
BP50, TNFRSF5
Isotype
Mouse IgG1, κ
HB14_Purified_062507
Human peripheral blood lymphocytes stained with purified HB14, followed by anti-mouse IgGs FITC
  • HB14_Purified_062507
    Human peripheral blood lymphocytes stained with purified HB14, followed by anti-mouse IgGs FITC
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Cat # Size Price Quantity Check Availability
313002 100 µg $101.00
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Description

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The HB14 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially block CD40 binding to CD40L and rescue B cells from apoptosis.

Technical data sheet

Product Details

Verified Reactivity
Human, Cynomolgus, Rhesus
Reported Reactivity
African Green, Baboon
Antibody Type
Monoclonal
Host Species
Mouse
Formulation
Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.
Preparation
The antibody was purified by affinity chromatography.
Concentration
0.5 mg/ml
Storage & Handling
The antibody solution should be stored undiluted between 2°C and 8°C.
Application

FC - Quality tested
Costim - Reported in the literature, not verified in house
Recommended Usage

Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.125 µg per million cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

Application Notes

Additional reported applications (for the relevant formats) include: costimulation of B cell proliferation, partial inhibition of CD40 binding to CD40L, and prevention of B cell apoptosis.1 Alone, or in combination with TLR ligands, clone HIB14 stimulates B cells to produce IL-10 and differentiates it into regulatory B10 (IL-10 producing B cells).7 The Ultra-LEAF™ purified antibody (Endotoxin <0.1 EU/µg, Azide-Free, 0.2 µm filtered) is recommended for functional assays (Cat. Nos. 313019 & 313020).

Application References

(PubMed link indicates BioLegend citation)
  1. Pound JD, et al. 1999. Int. Immunol. 11:11. (Costim)
  2. Schlossman S, et al. Eds. 1995. Leucocyte Typing V. Oxford University Press. New York.
  3. Armengol MP, et al. 2001. Am. J. Pathol. 159:861.
  4. Cavanagh LL, et al. 2005. Arthritis Res. Ther. 7:R230.
  5. Jayakumar A, et al. 2008. Infect Immun.76:2138. PubMed
  6. Sestak K, et al. 2007. Vet. Immunol. Immunopathol. 119:21.
  7. Iwata Y, et al. 2011. Blood. 117:530. PubMed
Product Citations
  1. Syrimi E, et al. 2021. iScience. 24:103215. PubMed
  2. Pissani F, et al. 2018. J Virol. 92:e01143. PubMed
  3. Jayakumar A, et al. 2008. Infect Immun. 76:2138. PubMed
  4. Kennedy-Darling J, et al. 2021. Eur J Immunol. 51:1262. PubMed
  5. Gonzalez-Figueroa P, et al. 2021. Cell. 184(7):1775-1789.e19. PubMed
RRID
AB_314965 (BioLegend Cat. No. 313002)

Antigen Details

Structure
TNFR superfamily, type I glycoprotein, 48 kD
Distribution

B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts

Function
B cell differentiation, costimulation, isotype class-switching, rescues B cells from apoptosis
Ligand/Receptor
CD154 (CD40 ligand)
Cell Type
B cells, Dendritic cells, Endothelial cells, Fibroblasts, Macrophages
Biology Area
Cell Biology, Costimulatory Molecules, Immunology, Neuroscience, Neuroscience Cell Markers
Molecular Family
CD Molecules
Antigen References

1. Banchereau J, et al. 1994. Annu. Rev. Immunol. 12:881.
2. Foy T, et al. 1996. Annu. Rev. Immunol. 14:591.

Gene ID
958 View all products for this Gene ID
UniProt
View information about CD40 on UniProt.org
Go To Top Version: 2    Revision Date: 08/25/2022

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